Projects per year
During my postdoctoral training in the Academic Medical Center of the University of Amsterdam, I have developed an ultra-sensitive HIV-1 detection assay, based on an innovative semi-nested qPCR of my own design (Pasternak, J Clin Microbiol 2008; Kiselinova, Pasternak, PLoS One 2014). This assay is extremely sensitive and robust, allowing to detect the virus in >90% of patient samples that are undetectable by conventional viral load measurements. This assay allowed us to identify cell-associated HIV RNA as a viral marker that predicts disease progression and therapy response, and we have demonstrated the clinical value of this biomarker in several publications. In particular, we have shown that cell-associated HIV RNA predicts antiretroviral therapy (ART) failure and correlates with subtle changes in patients’ adherence to ART in the absence of virological rebound in plasma (Pasternak, PLoS One 2009; J Infect Dis 2012). This pioneering work opened the door for a wider use of HIV biomarkers for monitoring ART response and studying HIV persistence, and the assays I developed are now used in a number of research laboratories worldwide, including those at the forefront of HIV cure research (Rasmussen, Lancet HIV 2014; Elliott, PLoS Pathogens 2014). More recently, I received two prestigious research grants from the Dutch AIDS Fonds as a Principal Investigator, allowing me to start an independent research group within the department. My current scientific interests are focused on persistence and dynamics of HIV reservoir. The ongoing research projects of my group deal with HIV reservoir and viral diversity in patients treated with ART during primary infection, viral and host factors determining immunological failure on ART, and virological correlates of co-morbidities in HIV-infected patients.
Expertise related to UN Sustainable Development Goals
In 2015, UN member states agreed to 17 global Sustainable Development Goals (SDGs) to end poverty, protect the planet and ensure prosperity for all. This person’s work contributes towards the following SDG(s):
Dive into details
Select a country/territory to view shared publications and projects
- 1 Active
Berkhout B.: Virology
Berkhout, B., Cornelissen, M., van der Kuyl, A., van Hemert, F., Herrera Carrillo, E., van Montfort, T., Pasternak, A. O., van der Velden, Y., Vink, M., Zorgdrager, F., Brouwer, P., Gao, Z., van Haaren, M., Del Moral Sanchez, I., Overmars, R., Schorcht, A., Steba, G., de Taeye, S., Torrents de la Pena, A., Wang, G. & Klaver, B.
1/01/2006 → …
Editorial: The relevance of molecular mechanisms in HIV-1 latency and reactivation from latencyPasternak, A. O., Rohr, O., van Lint, C. & Kula-Pacurar, A., 2023, In: Frontiers in cellular and infection microbiology. 13, 1190867.
Research output: Contribution to journal › Editorial › Academic › peer-reviewOpen Access
HIV persistence: silence or resistance?Pasternak, A. O. & Berkhout, B., 1 Apr 2023, In: Current opinion in virology. 59, 101301.
Research output: Contribution to journal › Review article › Academic › peer-reviewOpen Access1 Citation (Scopus)
Novel role of UHRF1 in the epigenetic repression of the latent HIV-1Verdikt, R., Bendoumou, M., Bouchat, S., Nestola, L., Pasternak, A. O., Darcis, G., Avettand-Fenoel, V. R., Vanhulle, C., Aït-Ammar, A., Santangelo, M., Plant, E., Douce, V. L., Delacourt, N., Cicilionytė, A., Necsoi, C., Corazza, F., Passaes, C. P. B., Schwartz, C., Bizet, M., Fuks, F., & 7 others, 1 May 2022, In: eBioMedicine. 79, 103985.
Research output: Contribution to journal › Article › Academic › peer-reviewOpen Access4 Citations (Scopus)
Towards a molecular profile of antiretroviral therapy-free HIV remissionAdams, P., Berkhout, B. & Pasternak, A. O., 1 Sept 2022, In: Current opinion in HIV and AIDS. 17, 5, p. 301-307 7 p.
Research output: Contribution to journal › Review article › Academic › peer-review1 Citation (Scopus)
Assessing proviral competence: current approaches to evaluate HIV-1 persistenceCicilionytė, A., Berkhout, B. & Pasternak, A. O., 1 Jul 2021, In: Current opinion in HIV and AIDS. 16, 4, p. 223-231 9 p.
Research output: Contribution to journal › Review article › Academic › peer-review6 Citations (Scopus)