Projects per year
Personal profile
Research interests
HIV replication and anti-viral strategies
This research line includes both basic and applied HIV research, which are intermingled and complement each other.
Basic research focuses on:
1. The structure and function of the HIV genome. In particular, we investigate the structure of the viral RNA and its role in different replication steps (such as transcription, RNA splicing, dimerization and packaging) and drug resistance.
2. The role of the viral Tat protein in HIV replication.
Applied research focuses on:
1. Novel strategies to inhibit HIV replication (based on the CRISPR/Cas9 system and RNA interference mechanism).
2. Development of conditionally replicating HIV variants as a novel approach toward a safe live-attenuated HIV vaccine and as a tool to study the host (immune) responses that correlate with protection induced by live-attenuated virus vaccines.
3. The use of viral evolution as a tool to develop new gene expression systems (Tet-On systems).
specialisation
Molecular biology of human immunodeficiency virus (HIV)
Expertise related to UN Sustainable Development Goals
In 2015, UN member states agreed to 17 global Sustainable Development Goals (SDGs) to end poverty, protect the planet and ensure prosperity for all. This person’s work contributes towards the following SDG(s):
External positions
Netherlands Commission on Genetic Modification (COGEM), Netherlands Commission on Genetic Modification (COGEM)
8 Jan 2018 → …
Collaborations and top research areas from the last five years
Recent external collaboration on country/territory level. Dive into details by clicking on the dots or
Projects
- 1 Active
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Das A.T.: HIV replication and anti-viral strategies
Das, A., Vink, M., Wang, G. & Klaver, B.
1/01/2008 → …
Project: Research
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HIV-1 3'-Polypurine Tract Mutations Confer Dolutegravir Resistance by Switching to an Integration-Independent Replication Mechanism via 1-LTR Circles
Dekker, J. G., Klaver, B., Berkhout, B. & Das, A. T., 31 May 2023, In: Journal of virology. 97, 5, p. e0036123Research output: Contribution to journal › Article › Academic › peer-review
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HIV co-opts a cellular antiviral mechanism, activation of stress kinase PKR by its RNA, to enable splicing of rev/tat mRNA
Namer, L. S., Harwig, A., Heynen, S. P., Das, A. T., Berkhout, B. & Kaempfer, R., 1 Dec 2023, In: Cell and Bioscience. 13, 1, 28.Research output: Contribution to journal › Article › Academic › peer-review
Open Access1 Citation (Scopus) -
Mutations in the HIV-1 3-Polypurine Tract Can Confer Dolutegravir Resistance
Dekker, J. G., Klaver, B., Berkhout, B. & Das, A. T., 1 Jan 2022, In: Antimicrobial Agents and Chemotherapy. 66, 1, e01027-21.Research output: Contribution to journal › Comment/Letter to the editor › Academic
7 Citations (Scopus) -
Transient crispr-cas treatment can prevent reactivation of hiv-1 replication in a latently infected t-cell line
Liu, Y., Jeeninga, R. E., Klaver, B., Berkhout, B. & Das, A. T., 1 Dec 2021, In: Viruses. 13, 12, 2461.Research output: Contribution to journal › Article › Academic › peer-review
Open Access4 Citations (Scopus) -
Variations in the abortive hiv-1 rna hairpin do not impede viral sensing and innate immune responses
Stunnenberg, M., van Hamme, J. L., Das, A. T., Berkhout, B. & Geijtenbeek, T. B. H., 1 Jul 2021, In: Pathogens (Basel, Switzerland). 10, 7, 897.Research output: Contribution to journal › Article › Academic › peer-review
Open Access1 Citation (Scopus)