Projects per year
I coordinated research efforts in Proteomics of human lysosomal storage disorders (LSD's), including implementation of ICAT-like techniques. I optimized the in gel digestion protocol and colloidal Coomassie staining to get routine identification at very low protein abundancies. I collaborated with many different groups (in and outside of the AMC) in Proteomic studies. In the context of my own research (a.o. Proteomics of HIV-Tcell interaction), I initiated the reproducible large scale DIGE approach in our lab (together with Jeffrey Ringrose and Rienk Jeeninga) and used this to monitor changes in cellular proteins induced by HIV-1 infection. In several collaborations I analysed changes in protein PTM's, identified several biologically important proteins, tested peptidomimetics for biological activities and was involved in immunologcal work. Lately, I have published several theoretical articles regarding the mitochondrial respiratory chain, genome architecture and (eukaryotic) evolution. I am also trying to start research to monitor oxidative damage of proteins, comparing conditions under which most energy is either derived from Fatty Acid or from Glucose breakdown, to test an hypothesis I first formulated in BioEssays 33, pp. 88-94. Last but not least, I have been extending this hypothesis, which might explain the evolution of peroxisomes and the absence of Fatty Acid oxidation in neurons, in several further publications focussing on the source of electrons entering the mitochondrial respiratory chain via NADH (complex I) or FADH2 (linked to several different complexes) and the resulting differences in ROS (reactive oxygen species) formation. I am currently collaborating with Antoine van Kampen (Medische Bioinformatica) to model these processes mathematically.
Expertise related to UN Sustainable Development Goals
In 2015, UN member states agreed to 17 global Sustainable Development Goals (SDGs) to end poverty, protect the planet and ensure prosperity for all. This person’s work contributes towards the following SDG(s):
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Aerts, J. M. F. G., Bleijlevens, B., Speijer, D., van Eijk, M. C., de Haan, A. J., Mirzaian, M., Ottenhoff, R., van Roomen, C. P. A. A., Scheij, S., Avagliano Trezza, R., Avula, T., Cook, E. C. L., Gkourtsa, A., Guimaraes da Lomba Ferraz, M. J., Herrera Moro Chao, H. M. C., Lizama Gabriel, T., Oussoren, S. V. & Wegdam, W.
1/01/2007 → …
Bruggeman, J. W., Koster, J., van Pelt, A. M. M., Speijer, D. & Hamer, G., Jan 2023, In: BioEssays. 45, 1, 2200112.
Research output: Contribution to journal › Article › Academic › peer-reviewOpen Access1 Citation (Scopus)
Speijer, D., Dec 2022, In: BioEssays. 44, 12, 2200189.
Research output: Contribution to journal › Editorial › Academic › peer-review
1 Citation (Scopus)
Hammond, M., Dorrell, R. G., Speijer, D. & Lukeš, J., May 2022, In: BioEssays. 44, 5, 2100258.
Research output: Contribution to Journal › Article › Academic › peer-review
Molecular characteristics of the multi-functional FAO enzyme ACAD9 illustrate the importance of FADH2/NADH ratios for mitochondrial ROS formationSpeijer, D., 1 Aug 2022, In: BioEssays. 44, 8, 2200056.
Research output: Contribution to journal › Article › Academic › peer-reviewOpen Access