Research output per year
Research output per year
Personal profile
specialisation
Cancer Genes · Oncogenetics · Cell Cycle and DNA Damage Repair Pathways · CRISPR Genomics
Research interests
- Identification of Novel Cancer Genes and Pathways
- Mechanisms Relevant for the Detection, Prevention and Treatment of Hereditary Cancer
- Factors Contributing to Genome Integrity and Cell Division
- Functional Genomics and CRISPR/Cas Applications
Rob Wolthuis is Associate Professor at the Department of Human Genetics, Amsterdam UMC. He is the Head of the Section of Oncogenetics and co-manager of the Amsterdam UMC CRISPR Expertise Center.
The Oncogenetics Section traditionally employs patient-derived cell models and their cDNA complemented counterparts to investigate hereditary cancer genes. Building on this foundation, they are engineering tissue-specific human cancer cell models by CRISPR/Cas-based gene editing now, to study cancer genes and associated pathways. The objectives are to understand the mechanisms underlying cancer formation, to identify disease markers and to pinpoint tumour vulnerabilities that could be exploited therapeutically.
A significant advancement in our research has been the development of genome-wide CRISPR screening pipelines in Cas9-inducible diploid human epithelial cells. This technique is instrumental in screening isogenic models of cancer genes. The resulting molecular genetic networks, thereby support the identification of potential biomarkers and targets for drug development.
Furthermore, Wolthuis's team is engaged in genome-wide screens in conjunction with targeted cancer drugs or relevant compounds. This research is critical in understanding genes underlying drug responses and reveal potential mechanisms of drug resistance. Obtained insights may also help to reveal disease prevention strategies for cancer predisposed individuals.
They are also pioneering the development of other CRISPR-related applications, e.g. the use of Cas12 in genome diagnostics. Looking ahead, there is an ambitious vision to explore the integration of targeted gene editing within clinically relevant therapeutic genetics.
Activities
- Associate Professor at the Department of Human Genetics, Amsterdam UMC
- Member of the Human Genetics Executive Daily Board
- Theme Leader in Cancer Biology, Cancer Center Amsterdam
- Manager Amsterdam UMC CRISPR Expertise Center
- Course Coordinator VU Master Oncology
External positions
Junior Group Leader, Netherlands Cancer Institute, Amsterdam, Netherlands
1 Jan 2007 → 31 Dec 2014
Postdoctoral Researcher, Netherlands Cancer Institute, Amsterdam, Netherlands
1 Jan 2004 → 31 Dec 2006
KWF Postdoctoral Fellow, University of Cambridge, Cambridge, United Kingdom
1 Jan 2000 → 31 Dec 2003
Junior Postdoc, UMC Utrecht, Utrecht, Netherlands
1 Feb 1998 → 31 Dec 1999
PhD Student, UMC Utrecht, Utrecht, Netherlands
1 Sept 1993 → 27 Jan 1998
Collaborations and top research areas from the last five years
Recent external collaboration on country/territory level. Dive into details by clicking on the dots or
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Correspondence on “Frequency of truncating FLCN variants and Birt-Hogg-Dubé-associated phenotypes in a health care system population” by Savatt et al
van Riel, L., Jansen, P. R., Boerrigter, B. G., van Moorselaar, R. J. A., van Haelst, M. M., Wolthuis, R. M. F., van de Beek, I. & Houweling, A. C., 1 Jan 2023, In: Genetics in medicine. 25, 1, p. 158-160 3 p.Research output: Contribution to journal › Comment/Letter to the editor › Academic
Open Access2 Citations (Scopus) -
CRISPR screens in sister chromatid cohesion defective cells reveal PAXIP1-PAGR1 as regulator of chromatin association of cohesin
van Schie, J. J. M., de Lint, K., Molenaar, T. M., Gines, M. M., Balk, J. A., Rooimans, M. A., Roohollahi, K., Pai, G. M., Borghuis, L., Ramadhin, A. R., Corazza, F., Dorsman, J. C., Wendt, K. S., Wolthuis, R. M. F. & de Lange, J., 13 Oct 2023, In: Nucleic Acids Research. 51, 18, p. 9594-9609 16 p.Research output: Contribution to journal › Article › Academic › peer-review
Open Access -
Familial multiple discoid fibromas is linked to a locus on chromosome 5 including the FNIP1 gene
van de Beek, I., Glykofridis, I. E., Tanck, M. W. T., Luijten, M. N. H., Starink, T. M., Balk, J. A., Johannesma, P. C., Hennekam, E., van den Hoff, M. J. B., Gunst, Q. D., Gille, J. J. P., Polstra, A. M., Postmus, P. E., van Steensel, M. A. M., Postma, A. V., Wolthuis, R. M. F., Menko, F. H., Houweling, A. C. & Waisfisz, Q., Apr 2023, In: Journal of Human Genetics. 68, 4, p. 273-279 7 p.Research output: Contribution to journal › Article › Academic › peer-review
Open Access3 Citations (Scopus) -
Genome-wide siRNA screens identify RBBP9 function as a potential target in Fanconi anaemia-deficient head-and-neck squamous cell carcinoma
Pai, G., Roohollahi, K., Rockx, D., de Jong, Y., Stoepker, C., Pennings, C., Rooimans, M., Vriend, L., Piersma, S., Jimenez, C. R., De Menezes, R. X., Van Beusechem, V. W., Brakenhoff, R. H., Te Riele, H., Wolthuis, R. M. F. & Dorsman, J. C., 1 Dec 2023, In: Communications Biology. 6, 1, p. 37 37.Research output: Contribution to journal › Article › Academic › peer-review
Open Access -
In Vitro CRISPR-Cas12a-Based Detection of Cancer-Associated TP53 Hotspot Mutations Beyond the crRNA Seed Region
Kohabir, KAV., Nooi, L., Brink, A., Brakenhoff, RH., Sistermans, EA. & Wolthuis, RMF., 1 Apr 2023, In: The CRISPR Journal. 6, 2, p. 127-139 13 p.Research output: Contribution to journal › Article › Academic › peer-review
Open Access1 Citation (Scopus)