TY - JOUR
T1 - The endometrial microbiota of women with or without a live birth within 12 months after a first failed IVF/ICSI cycle
AU - Bui, Bich Ngoc
AU - van Hoogenhuijze, Nienke
AU - Viveen, Marco
AU - Mol, Femke
AU - Teklenburg, Gijs
AU - de Bruin, Jan-Peter
AU - Besselink, Dagmar
AU - Brentjens, Linda Stevens
AU - Mackens, Shari
AU - Rogers, Malbert R. C.
AU - Steba, Gaby S.
AU - Broekmans, Frank
AU - Paganelli, Fernanda L.
AU - van de Wijgert, Janneke H. H. M.
N1 - Funding Information: The randomised controlled trial was funded by the Netherlands Organisation for Health Research and Development, ‘ZonMw’ (ZonMW project number 843002601). The current microbiota project was funded by Merck and the Utrecht Exposome Hub of Utrecht Life Sciences ( www.uu.nl/exposome ), which was funded by the Executive Board of Utrecht University. None of the study donors had input in the study design, analysis, or data interpretation. Publisher Copyright: © 2023, The Author(s).
PY - 2023/12/1
Y1 - 2023/12/1
N2 - The endometrial microbiota composition may be associated with implantation success. However, a ‘core’ composition has not yet been defined. This exploratory study analysed the endometrial microbiota by 16S rRNA sequencing (V1–V2 region) of 141 infertile women whose first IVF/ICSI cycle failed and compared the microbiota profiles of women with and without a live birth within 12 months of follow-up, and by infertility cause and type. Lactobacillus was the most abundant genus in the majority of samples. Women with a live birth compared to those without had significantly higher Lactobacillus crispatus relative abundance (RA) (p = 0.029), and a smaller proportion of them had ≤ 10% L. crispatus RA (42.1% and 70.4%, respectively; p = 0.015). A smaller proportion of women in the male factor infertility group had ≤ 10% L. crispatus RA compared to women in the unexplained and other infertility causes groups combined (p = 0.030). Women with primary infertility compared to secondary infertility had significantly higher L. crispatus RA (p = 0.004); lower proportions of them had ≤ 10% L. crispatus RA (p = 0.009) and > 10% Gardnerella vaginalis RA (p = 0.019). In conclusion, IVF/ICSI success may be associated with L. crispatus RA and secondary infertility with endometrial dysbiosis, more often than primary infertility. These hypotheses should be tested in rigorous well-powered longitudinal studies.
AB - The endometrial microbiota composition may be associated with implantation success. However, a ‘core’ composition has not yet been defined. This exploratory study analysed the endometrial microbiota by 16S rRNA sequencing (V1–V2 region) of 141 infertile women whose first IVF/ICSI cycle failed and compared the microbiota profiles of women with and without a live birth within 12 months of follow-up, and by infertility cause and type. Lactobacillus was the most abundant genus in the majority of samples. Women with a live birth compared to those without had significantly higher Lactobacillus crispatus relative abundance (RA) (p = 0.029), and a smaller proportion of them had ≤ 10% L. crispatus RA (42.1% and 70.4%, respectively; p = 0.015). A smaller proportion of women in the male factor infertility group had ≤ 10% L. crispatus RA compared to women in the unexplained and other infertility causes groups combined (p = 0.030). Women with primary infertility compared to secondary infertility had significantly higher L. crispatus RA (p = 0.004); lower proportions of them had ≤ 10% L. crispatus RA (p = 0.009) and > 10% Gardnerella vaginalis RA (p = 0.019). In conclusion, IVF/ICSI success may be associated with L. crispatus RA and secondary infertility with endometrial dysbiosis, more often than primary infertility. These hypotheses should be tested in rigorous well-powered longitudinal studies.
UR - http://www.scopus.com/inward/record.url?scp=85149422847&partnerID=8YFLogxK
U2 - https://doi.org/10.1038/s41598-023-30591-2
DO - https://doi.org/10.1038/s41598-023-30591-2
M3 - Article
C2 - 36859567
SN - 2045-2322
VL - 13
JO - Scientific reports
JF - Scientific reports
IS - 1
M1 - 3444
ER -