@article{2649a38ad2934caf9526977ea9aab5a6,
title = "Intestinal organoid co-culture protocol to study cell competition in vitro",
abstract = "Intestinal organoid cultures are a powerful tool to study epithelial cells in vitro, as they are able to proliferate and differentiate into all cell lineages observed in vivo. Co-culturing organoids with distinct genetic backgrounds provides an excellent approach to study contact dependent and independent interactions between healthy and mutant epithelial intestinal cells. Here, we provide 2D and 3D approaches to mouse organoid co-cultures using fluorescently labeled organoids and demonstrate the analysis of these co-cultures using flow cytometry and microscopy-based approaches. For complete details on the use and execution of this profile, please refer to van Neerven et al., 2021.",
keywords = "Cancer, Cell Biology, Cell culture, Cell-based Assays, Flow Cytometry/Mass Cytometry, Microscopy, Organoids",
author = "{van Neerven}, {Sanne M.} and Rana Ramadan and {van Driel}, {Milou S.} and Huels, {David J.} and Louis Vermeulen",
note = "Funding Information: S.M.v.N. is supported by a NWO OOA PhD scholarship ( 022.005.002 ). This work is supported by The New York Stem Cell Foundation and grants from KWF ( UVA2014-7245 ), the Maurits en Anna de Kock Stichting ( 2015-2 ), Worldwide Cancer Research ( 14-1164 ), the Maag Lever Darm Stichting ( MLDS-CDG 14-03 ), the European Research Council ( ERG-StG 638193 ), and ZonMw ( Vidi 016.156.308 ) to L.V. D.J.H. is supported by the KWF Young Investigator program ( 13544 ). L.V. is a New York Stem Cell Foundation –Robertson Investigator. Publisher Copyright: {\textcopyright} 2021 The Author(s)",
year = "2022",
month = mar,
day = "18",
doi = "https://doi.org/10.1016/j.xpro.2021.101050",
language = "English",
volume = "3",
journal = "STAR Protocols",
issn = "2666-1667",
publisher = "Cell Press",
number = "1",
}