TY - JOUR
T1 - Crosstalk between R848 and abortive HIV-1 RNA-induced signaling enhances antiviral immunity
AU - Stunnenberg, Melissa
AU - van Hamme, John L.
AU - Zijlstra-Willems, Esther M.
AU - Gringhuis, Sonja I.
AU - Geijtenbeek, Teunis B. H.
N1 - Funding Information: This work was supported by Aidsfonds (P‐9906) and the European Research Council (Advanced grant 670424). Publisher Copyright: © 2022 The Authors. Journal of Leukocyte Biology published by Wiley Periodicals LLC on behalf of Society for Leukocyte Biology.
PY - 2022/8
Y1 - 2022/8
N2 - Pathogens trigger multiple pattern recognition receptors (PRRs) that together dictate innate and adaptive immune responses. Understanding the crosstalk between PRRs is important to enhance vaccine efficacy. Abortive HIV-1 RNA transcripts are produced during acute and chronic HIV-1 infection and are known ligands for different PRRs, leading to antiviral and proinflammatory responses. Here, we have investigated the crosstalk between responses induced by these 58 nucleotide-long HIV-1 RNA transcripts and different TLR ligands. Costimulation of dendritic cells (DCs) with abortive HIV-1 RNA and TLR7/8 agonist R848, but not other TLR agonists, resulted in enhanced antiviral type I IFN responses as well as adaptive immune responses via the induction of DC-mediated T helper 1 (TH1) responses and IFNγ+CD8+ T cells. Our data underscore the importance of crosstalk between abortive HIV-1 RNA and R848-induced signaling for the induction of effective antiviral immunity.
AB - Pathogens trigger multiple pattern recognition receptors (PRRs) that together dictate innate and adaptive immune responses. Understanding the crosstalk between PRRs is important to enhance vaccine efficacy. Abortive HIV-1 RNA transcripts are produced during acute and chronic HIV-1 infection and are known ligands for different PRRs, leading to antiviral and proinflammatory responses. Here, we have investigated the crosstalk between responses induced by these 58 nucleotide-long HIV-1 RNA transcripts and different TLR ligands. Costimulation of dendritic cells (DCs) with abortive HIV-1 RNA and TLR7/8 agonist R848, but not other TLR agonists, resulted in enhanced antiviral type I IFN responses as well as adaptive immune responses via the induction of DC-mediated T helper 1 (TH1) responses and IFNγ+CD8+ T cells. Our data underscore the importance of crosstalk between abortive HIV-1 RNA and R848-induced signaling for the induction of effective antiviral immunity.
KW - TLR8
KW - adaptive immunity
KW - immunomodulation
KW - innate immunity
KW - pathogen-associated molecular pattern
KW - pattern recognition receptor
UR - http://www.scopus.com/inward/record.url?scp=85122265722&partnerID=8YFLogxK
U2 - https://doi.org/10.1002/JLB.4A0721-365R
DO - https://doi.org/10.1002/JLB.4A0721-365R
M3 - Article
C2 - 34982481
SN - 0741-5400
VL - 112
SP - 289
EP - 298
JO - Journal of leukocyte biology
JF - Journal of leukocyte biology
IS - 2
ER -