A high-dimensional window into the micro-environment of triple negative breast cancer

Iris Nederlof; Hugo M. Horlings; Christina Curtis; Marleen Kok

doi:https://doi.org/10.3390/cancers13020316

A high-dimensional window into the micro-environment of triple negative breast cancer

Iris Nederlof, Hugo M. Horlings, Christina Curtis, Marleen Kok

Research output: Contribution to journal › Article › Academic › peer-review

13 Citations (Scopus)

Abstract

Providing effective personalized immunotherapy for triple negative breast cancer (TNBC) patients requires a detailed understanding of the composition of the tumor microenvironment. Both the tumor cell and non-tumor components of TNBC can exhibit tremendous heterogeneity in individual patients and change over time. Delineating cellular phenotypes and spatial topographies associated with distinct immunological states and the impact of chemotherapy will be necessary to optimally time immunotherapy. The clinical successes in immunotherapy have intensified research on the tumor microenvironment, aided by a plethora of high-dimensional technologies to define cellular phenotypes. These high-dimensional technologies include, but are not limited to, single cell RNA sequencing, spatial transcriptomics, T cell repertoire analyses, advanced flow cytometry, imaging mass cytometry, and their integration. In this review, we discuss the cellular phenotypes and spatial patterns of the lymphoid-, myeloid-, and stromal cells in the TNBC microenvironment and the potential value of mapping these features onto tumor cell genotypes.

Original language	English
Article number	316
Pages (from-to)	1-19
Number of pages	19
Journal	Cancers
Volume	13
Issue number	2
DOIs	https://doi.org/10.3390/cancers13020316
Publication status	Published - 2 Jan 2021

Keywords

Breast cancer
Immune profiling
Single cell
Spatial profiling
Tumor evolution

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https://doi.org/10.3390/cancers13020316

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title = "A high-dimensional window into the micro-environment of triple negative breast cancer",

abstract = "Providing effective personalized immunotherapy for triple negative breast cancer (TNBC) patients requires a detailed understanding of the composition of the tumor microenvironment. Both the tumor cell and non-tumor components of TNBC can exhibit tremendous heterogeneity in individual patients and change over time. Delineating cellular phenotypes and spatial topographies associated with distinct immunological states and the impact of chemotherapy will be necessary to optimally time immunotherapy. The clinical successes in immunotherapy have intensified research on the tumor microenvironment, aided by a plethora of high-dimensional technologies to define cellular phenotypes. These high-dimensional technologies include, but are not limited to, single cell RNA sequencing, spatial transcriptomics, T cell repertoire analyses, advanced flow cytometry, imaging mass cytometry, and their integration. In this review, we discuss the cellular phenotypes and spatial patterns of the lymphoid-, myeloid-, and stromal cells in the TNBC microenvironment and the potential value of mapping these features onto tumor cell genotypes.",

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note = "Funding Information: I.N. is supported by the Dutch Cancer Foundation (KWF-10510). C.C. is supported by the National Institutes of Health (NIH) through the NIH Director?s Pioneer Award (DP1-CA238296) and R01CA182514, as well as the Breast Cancer Research Foundation. H.M.H. reports funding to the institute from Roche/Genentech. M. K. reports funding to the institute from BMS, Roche/Genentech, AZ. Figure 1 was partially created with BioRender.com. Publisher Copyright: {\textcopyright} 2021 by the authors.",

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A high-dimensional window into the micro-environment of triple negative breast cancer

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Keywords

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