TY - JOUR
T1 - A method comparison of total and HMW adiponectin: HMW/total adiponectin ratio varies versus total adiponectin, independent of clinical condition
T2 - HMW/total adiponectin ratio varies versus total adiponectin, independent of clinical condition
AU - van Andel, Merel
AU - Drent, Madeleine L.
AU - van Herwaarden, Antonius E.
AU - Ackermans, Mariëtte T.
AU - Heijboer, Annemieke C.
PY - 2017/2/1
Y1 - 2017/2/1
N2 - Background: Total and high-molecular-weight (HMW) adiponectin have been associated with endocrine and cardiovascular pathology. As no gold standard is available, the discussion about biological relevance of isoforms is complicated. In our study we perform a method comparison between two commercially available assays measuring HMW and total adiponectin, in various patient groups, thus contributing further to this discussion. Methods: We determined levels of HMW and total adiponectin using assays by Lumipulse (R) and Millipore (R) respectively, in 126 patients with different clinical characteristics (n = 29 healthy volunteers, n = 22 dialysis patients, n = 25 elderly with body mass index (BMI) <21 kg/m(2), n = 25 elderly with BMI 30-35 kg/m2, n = 26 children). Results: The Passing & Bablock regression analysis resulted in HMW adiponectin(LUMIPULSE) * 0.5 - 0.9 = total adiponectin(LUMIPULSE), albeit with significant deviation from linearity (p <0.001). Pearson's correlation was R = 0.987 (p = 0.000). No significant differences between patient groups were observed (p = 0.190). Conclusions: The HMW/total adiponectin ratio varies with total adiponectin concentration independent of clinical conditions studied. Our results imply that total and HMW adiponectin have similar utility when assessing adiponectin levels in blood, as the ratio is independent of clinical condition. (C) 2016 Elsevier B.V. All rights reserved
AB - Background: Total and high-molecular-weight (HMW) adiponectin have been associated with endocrine and cardiovascular pathology. As no gold standard is available, the discussion about biological relevance of isoforms is complicated. In our study we perform a method comparison between two commercially available assays measuring HMW and total adiponectin, in various patient groups, thus contributing further to this discussion. Methods: We determined levels of HMW and total adiponectin using assays by Lumipulse (R) and Millipore (R) respectively, in 126 patients with different clinical characteristics (n = 29 healthy volunteers, n = 22 dialysis patients, n = 25 elderly with body mass index (BMI) <21 kg/m(2), n = 25 elderly with BMI 30-35 kg/m2, n = 26 children). Results: The Passing & Bablock regression analysis resulted in HMW adiponectin(LUMIPULSE) * 0.5 - 0.9 = total adiponectin(LUMIPULSE), albeit with significant deviation from linearity (p <0.001). Pearson's correlation was R = 0.987 (p = 0.000). No significant differences between patient groups were observed (p = 0.190). Conclusions: The HMW/total adiponectin ratio varies with total adiponectin concentration independent of clinical conditions studied. Our results imply that total and HMW adiponectin have similar utility when assessing adiponectin levels in blood, as the ratio is independent of clinical condition. (C) 2016 Elsevier B.V. All rights reserved
KW - Adiponectin isoforms
KW - Cardiovascular disease
KW - High molecular weight/total adiponectin ratio
KW - Method comparison
KW - Patient characteristics
UR - http://www.scopus.com/inward/record.url?scp=85006166031&partnerID=8YFLogxK
U2 - https://doi.org/10.1016/j.cca.2016.12.009
DO - https://doi.org/10.1016/j.cca.2016.12.009
M3 - Article
C2 - 27986548
SN - 0009-8981
VL - 465
SP - 30
EP - 33
JO - Clinica chimica acta; international journal of clinical chemistry
JF - Clinica chimica acta; international journal of clinical chemistry
ER -