TY - JOUR
T1 - A novel genus in the order Picornavirales detected in human stool
AU - Oude Munnink, Bas B.
AU - Cotten, Matthew
AU - Deijs, Martin
AU - Jebbink, Maarten F.
AU - Bakker, Margreet
AU - Farsani, Seyed Mohammad Jazaeri
AU - Canuti, Marta
AU - Kellam, Paul
AU - van der Hoek, Lia
PY - 2015
Y1 - 2015
N2 - Recently, four new viruses belonging to an unassigned family within the order Picornavirales were identified in excrements of healthy carp (fisavirus) and pigs (posavirus 1, 2 and 3). We report the detection and characterization of a fifth virus present in human faeces. The virus, named human stool-associated RNA virus (husavirus), contains a single ORF encoding a putative 2993 AA polyprotein, with a Hel-Pro-Pol replication block, typical for the Picornavirales. Phylogenetic analysis revealed that the closest relative to husavirus is posavirus 1, and together they cluster with fisavirus, posavirus 2 and 3 and a roundworm (Ascaris suum) derived virus. Husavirus was detected in eight human stool samples collected in 1984 (n52), 1985 (n54), 1995 (n51) and 2014 (n51). From three strains of husavirus from 1984 and 1985 the full genome sequence was determined, showing less than 5% intraspecies variation in the nucleotide composition. The host of this virus remains to be determined
AB - Recently, four new viruses belonging to an unassigned family within the order Picornavirales were identified in excrements of healthy carp (fisavirus) and pigs (posavirus 1, 2 and 3). We report the detection and characterization of a fifth virus present in human faeces. The virus, named human stool-associated RNA virus (husavirus), contains a single ORF encoding a putative 2993 AA polyprotein, with a Hel-Pro-Pol replication block, typical for the Picornavirales. Phylogenetic analysis revealed that the closest relative to husavirus is posavirus 1, and together they cluster with fisavirus, posavirus 2 and 3 and a roundworm (Ascaris suum) derived virus. Husavirus was detected in eight human stool samples collected in 1984 (n52), 1985 (n54), 1995 (n51) and 2014 (n51). From three strains of husavirus from 1984 and 1985 the full genome sequence was determined, showing less than 5% intraspecies variation in the nucleotide composition. The host of this virus remains to be determined
U2 - https://doi.org/10.1099/jgv.0.000279
DO - https://doi.org/10.1099/jgv.0.000279
M3 - Article
C2 - 26354795
SN - 0022-1317
VL - 96
SP - 3440
EP - 3443
JO - Journal of general virology
JF - Journal of general virology
IS - 11
ER -