A rapid and potent DNA vaccination strategy defined by in vivo monitoring of antigen expression

Adriaan D. Bins; Annelies Jorritsma; Monika C. Wolkers; Chien-Fu Hung; T.-C. Wu; Ton N. M. Schumacher; John B. A. G. Haanen

doi:https://doi.org/10.1038/nm1264

A rapid and potent DNA vaccination strategy defined by in vivo monitoring of antigen expression

Adriaan D. Bins, Annelies Jorritsma, Monika C. Wolkers, Chien-Fu Hung, T.-C. Wu, Ton N. M. Schumacher, John B. A. G. Haanen

Center of Experimental and Molecular Medicine (AMC)

Research output: Contribution to journal › Article › Academic › peer-review

148 Citations (Scopus)

Abstract

Induction of immunity after DNA vaccination is generally considered a slow process. Here we show that DNA delivery to the skin results in a highly transient pulse of antigen expression. Based on this information, we developed a new rapid and potent intradermal DNA vaccination method. By short-interval intradermal DNA delivery, robust T-cell responses, of a magnitude sufficient to reject established subcutaneous tumors, are generated within 12 d. Moreover, this vaccination strategy confers protecting humoral immunity against influenza A infection within 2 weeks after the start of vaccination. The strength and speed of this newly developed strategy will be beneficial in situations in which immunity is required in the shortest possible time

Original language	English
Pages (from-to)	899-904
Journal	Nature medicine
Volume	11
Issue number	8
DOIs	https://doi.org/10.1038/nm1264
Publication status	Published - 2005

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https://doi.org/10.1038/nm1264

Cite this

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title = "A rapid and potent DNA vaccination strategy defined by in vivo monitoring of antigen expression",

abstract = "Induction of immunity after DNA vaccination is generally considered a slow process. Here we show that DNA delivery to the skin results in a highly transient pulse of antigen expression. Based on this information, we developed a new rapid and potent intradermal DNA vaccination method. By short-interval intradermal DNA delivery, robust T-cell responses, of a magnitude sufficient to reject established subcutaneous tumors, are generated within 12 d. Moreover, this vaccination strategy confers protecting humoral immunity against influenza A infection within 2 weeks after the start of vaccination. The strength and speed of this newly developed strategy will be beneficial in situations in which immunity is required in the shortest possible time",

author = "Bins, {Adriaan D.} and Annelies Jorritsma and Wolkers, {Monika C.} and Chien-Fu Hung and T.-C. Wu and Schumacher, {Ton N. M.} and Haanen, {John B. A. G.}",

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AU - Bins, Adriaan D.

AU - Jorritsma, Annelies

AU - Wolkers, Monika C.

AU - Hung, Chien-Fu

AU - Wu, T.-C.

AU - Schumacher, Ton N. M.

AU - Haanen, John B. A. G.

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