TY - JOUR
T1 - A two-gene methylation signature for the diagnosis of bladder cancer in urine
AU - Bosschieter, Judith
AU - Nieuwenhuijzen, Jakko A.
AU - Hentschel, Anouk
AU - van Splunter, Annina P.
AU - Segerink, Loes I.
AU - Vis, André N.
AU - Wilting, Saskia M.
AU - Lissenberg-Witte, Birgit I.
AU - A van Moorselaar, R. Jeroen
AU - Steenbergen, Renske D. M.
PY - 2019/2/1
Y1 - 2019/2/1
N2 - Aim: To analyze the potential of 14 cancer-associated genes, including six miRNAs, for bladder cancer (BC) diagnosis in urine. Patients & methods: DNA methylation levels of 14 genes were analyzed in urine of 72 BC patients and 75 healthy controls using quantitative methylation-specific PCR. Multivariate logistic regression analysis was used to determine an optimal marker panel. Results: Ten genes were significantly hypermethylated in BC patients. The GHSR/MAL combination showed the best diagnostic performance, reaching a sensitivity of 92% (95% CI: 86-99) and a specificity of 85% (95% CI: 76-94). Conclusion: We identified a novel two-gene panel with a high diagnostic accuracy for BC that can be applied in a noninvasive, urine-based test.
AB - Aim: To analyze the potential of 14 cancer-associated genes, including six miRNAs, for bladder cancer (BC) diagnosis in urine. Patients & methods: DNA methylation levels of 14 genes were analyzed in urine of 72 BC patients and 75 healthy controls using quantitative methylation-specific PCR. Multivariate logistic regression analysis was used to determine an optimal marker panel. Results: Ten genes were significantly hypermethylated in BC patients. The GHSR/MAL combination showed the best diagnostic performance, reaching a sensitivity of 92% (95% CI: 86-99) and a specificity of 85% (95% CI: 76-94). Conclusion: We identified a novel two-gene panel with a high diagnostic accuracy for BC that can be applied in a noninvasive, urine-based test.
KW - biomarkers
KW - methylation
KW - molecular diagnostics
KW - urinary bladder neoplasms
KW - urine analysis
UR - https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85061513673&origin=inward
UR - https://www.ncbi.nlm.nih.gov/pubmed/30706728
UR - http://www.scopus.com/inward/record.url?scp=85061513673&partnerID=8YFLogxK
U2 - https://doi.org/10.2217/epi-2018-0094
DO - https://doi.org/10.2217/epi-2018-0094
M3 - Article
C2 - 30706728
SN - 1750-1911
VL - 11
SP - 337
EP - 347
JO - Epigenomics
JF - Epigenomics
IS - 3
ER -