Absence of host-specific genes in canine and human staphylococcus pseudintermedius as inferred from comparative genomics

Alice Wegener; Els M. Broens; Linda van der Graaf-van Bloois; Aldert L. Zomer; Caroline E. Visser; Jan van Zeijl; Coby van der Meer; Johannes G. Kusters; Alex W. Friedrich; Greetje A. Kampinga; Gregorius J. Sips; Leonard Smeets; Manfred E. J. van Kerckhoven; Arjen J. Timmerman; Jaap A. Wagenaar; Birgitta Duim

doi:https://doi.org/10.3390/antibiotics10070854

Absence of host-specific genes in canine and human staphylococcus pseudintermedius as inferred from comparative genomics

Alice Wegener, Els M. Broens, Linda van der Graaf-van Bloois, Aldert L. Zomer, Caroline E. Visser, Jan van Zeijl, Coby van der Meer, Johannes G. Kusters, Alex W. Friedrich, Greetje A. Kampinga, Gregorius J. Sips, Leonard Smeets, Manfred E. J. van Kerckhoven, Arjen J. Timmerman, Jaap A. Wagenaar, Birgitta Duim

Medical Microbiology and Infection Prevention (AMC)

Research output: Contribution to journal › Article › Academic › peer-review

8 Citations (Scopus)

Abstract

Staphylococcus pseudintermedius is an important pathogen in dogs that occasionally causes infections in humans as an opportunistic pathogen of elderly and immunocompromised people. This study compared the genomic relatedness and antimicrobial resistance genes using genome-wide association study (GWAS) to examine host association of canine and human S. pseudintermedius isolates. Canine (n = 25) and human (n = 32) methicillin-susceptible S. pseudintermedius (MSSP) isolates showed a high level of genetic diversity with an overrepresentation of clonal complex CC241 in human isolates. This clonal complex was associated with carriage of a plasmid containing a bacteriocin with cytotoxic properties, a CRISPR-cas domain and a pRE25-like mobile element containing five antimicrobial resistance genes. Multi-drug resistance (MDR) was predicted in 13 (41%) of human isolates and 14 (56%) of canine isolates. CC241 represented 54% of predicted MDR isolates from humans and 21% of predicted MDR canine isolates. While it had previously been suggested that certain host-specific genes were present the current GWAS analysis did not identify any genes that were significantly associated with human or canine isolates. In conclusion, this is the first genomic study showing that MSSP is genetically diverse in both hosts and that multidrug resistance is important in dog and human-associated S. pseudintermedius isolates.

Original language	English
Article number	854
Journal	Antibiotics (Basel, Switzerland)
Volume	10
Issue number	7
DOIs	https://doi.org/10.3390/antibiotics10070854
Publication status	Published - 1 Jul 2021

Keywords

Antimicrobial resistance
Comparative genomics
Host association
S. pseudintermedius

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Wegener, A., Broens, E. M., van der Graaf-van Bloois, L., Zomer, A. L., Visser, C. E., van Zeijl, J., van der Meer, C., Kusters, J. G., Friedrich, A. W., Kampinga, G. A., Sips, G. J., Smeets, L., van Kerckhoven, M. E. J., Timmerman, A. J., Wagenaar, J. A., & Duim, B. (2021). Absence of host-specific genes in canine and human staphylococcus pseudintermedius as inferred from comparative genomics. Antibiotics (Basel, Switzerland), 10(7), Article 854. https://doi.org/10.3390/antibiotics10070854

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title = "Absence of host-specific genes in canine and human staphylococcus pseudintermedius as inferred from comparative genomics",

abstract = "Staphylococcus pseudintermedius is an important pathogen in dogs that occasionally causes infections in humans as an opportunistic pathogen of elderly and immunocompromised people. This study compared the genomic relatedness and antimicrobial resistance genes using genome-wide association study (GWAS) to examine host association of canine and human S. pseudintermedius isolates. Canine (n = 25) and human (n = 32) methicillin-susceptible S. pseudintermedius (MSSP) isolates showed a high level of genetic diversity with an overrepresentation of clonal complex CC241 in human isolates. This clonal complex was associated with carriage of a plasmid containing a bacteriocin with cytotoxic properties, a CRISPR-cas domain and a pRE25-like mobile element containing five antimicrobial resistance genes. Multi-drug resistance (MDR) was predicted in 13 (41%) of human isolates and 14 (56%) of canine isolates. CC241 represented 54% of predicted MDR isolates from humans and 21% of predicted MDR canine isolates. While it had previously been suggested that certain host-specific genes were present the current GWAS analysis did not identify any genes that were significantly associated with human or canine isolates. In conclusion, this is the first genomic study showing that MSSP is genetically diverse in both hosts and that multidrug resistance is important in dog and human-associated S. pseudintermedius isolates.",

keywords = "Antimicrobial resistance, Comparative genomics, Host association, S. pseudintermedius",

author = "Alice Wegener and Broens, {Els M.} and {van der Graaf-van Bloois}, Linda and Zomer, {Aldert L.} and Visser, {Caroline E.} and {van Zeijl}, Jan and {van der Meer}, Coby and Kusters, {Johannes G.} and Friedrich, {Alex W.} and Kampinga, {Greetje A.} and Sips, {Gregorius J.} and Leonard Smeets and {van Kerckhoven}, {Manfred E. J.} and Timmerman, {Arjen J.} and Wagenaar, {Jaap A.} and Birgitta Duim",

note = "Publisher Copyright: {\textcopyright} 2021 by the authors. Licensee MDPI, Basel, Switzerland.",

year = "2021",

month = jul,

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doi = "https://doi.org/10.3390/antibiotics10070854",

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Wegener, A, Broens, EM, van der Graaf-van Bloois, L, Zomer, AL, Visser, CE, van Zeijl, J, van der Meer, C, Kusters, JG, Friedrich, AW, Kampinga, GA, Sips, GJ, Smeets, L, van Kerckhoven, MEJ, Timmerman, AJ, Wagenaar, JA & Duim, B 2021, 'Absence of host-specific genes in canine and human staphylococcus pseudintermedius as inferred from comparative genomics', Antibiotics (Basel, Switzerland), vol. 10, no. 7, 854. https://doi.org/10.3390/antibiotics10070854

TY - JOUR

T1 - Absence of host-specific genes in canine and human staphylococcus pseudintermedius as inferred from comparative genomics

AU - Wegener, Alice

AU - Broens, Els M.

AU - van der Graaf-van Bloois, Linda

AU - Zomer, Aldert L.

AU - Visser, Caroline E.

AU - van Zeijl, Jan

AU - van der Meer, Coby

AU - Kusters, Johannes G.

AU - Friedrich, Alex W.

AU - Kampinga, Greetje A.

AU - Sips, Gregorius J.

AU - Smeets, Leonard

AU - van Kerckhoven, Manfred E. J.

AU - Timmerman, Arjen J.

AU - Wagenaar, Jaap A.

AU - Duim, Birgitta

PY - 2021/7/1

Y1 - 2021/7/1

N2 - Staphylococcus pseudintermedius is an important pathogen in dogs that occasionally causes infections in humans as an opportunistic pathogen of elderly and immunocompromised people. This study compared the genomic relatedness and antimicrobial resistance genes using genome-wide association study (GWAS) to examine host association of canine and human S. pseudintermedius isolates. Canine (n = 25) and human (n = 32) methicillin-susceptible S. pseudintermedius (MSSP) isolates showed a high level of genetic diversity with an overrepresentation of clonal complex CC241 in human isolates. This clonal complex was associated with carriage of a plasmid containing a bacteriocin with cytotoxic properties, a CRISPR-cas domain and a pRE25-like mobile element containing five antimicrobial resistance genes. Multi-drug resistance (MDR) was predicted in 13 (41%) of human isolates and 14 (56%) of canine isolates. CC241 represented 54% of predicted MDR isolates from humans and 21% of predicted MDR canine isolates. While it had previously been suggested that certain host-specific genes were present the current GWAS analysis did not identify any genes that were significantly associated with human or canine isolates. In conclusion, this is the first genomic study showing that MSSP is genetically diverse in both hosts and that multidrug resistance is important in dog and human-associated S. pseudintermedius isolates.

AB - Staphylococcus pseudintermedius is an important pathogen in dogs that occasionally causes infections in humans as an opportunistic pathogen of elderly and immunocompromised people. This study compared the genomic relatedness and antimicrobial resistance genes using genome-wide association study (GWAS) to examine host association of canine and human S. pseudintermedius isolates. Canine (n = 25) and human (n = 32) methicillin-susceptible S. pseudintermedius (MSSP) isolates showed a high level of genetic diversity with an overrepresentation of clonal complex CC241 in human isolates. This clonal complex was associated with carriage of a plasmid containing a bacteriocin with cytotoxic properties, a CRISPR-cas domain and a pRE25-like mobile element containing five antimicrobial resistance genes. Multi-drug resistance (MDR) was predicted in 13 (41%) of human isolates and 14 (56%) of canine isolates. CC241 represented 54% of predicted MDR isolates from humans and 21% of predicted MDR canine isolates. While it had previously been suggested that certain host-specific genes were present the current GWAS analysis did not identify any genes that were significantly associated with human or canine isolates. In conclusion, this is the first genomic study showing that MSSP is genetically diverse in both hosts and that multidrug resistance is important in dog and human-associated S. pseudintermedius isolates.

KW - Antimicrobial resistance

KW - Comparative genomics

KW - Host association

KW - S. pseudintermedius

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U2 - https://doi.org/10.3390/antibiotics10070854

DO - https://doi.org/10.3390/antibiotics10070854

M3 - Article

C2 - 34356775

SN - 2079-6382

VL - 10

JO - Antibiotics (Basel, Switzerland)

JF - Antibiotics (Basel, Switzerland)

IS - 7

M1 - 854

ER -

Absence of host-specific genes in canine and human staphylococcus pseudintermedius as inferred from comparative genomics

Abstract

Keywords

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