Aging selectively dampens oscillation of lipid abundance in white and brown adipose tissue

Ntsiki M. Held, M. Renate Buijink, Hyung L. Elfrink, Sander Kooijman, Georges E. Janssens, Angela C. M. Luyf, Mia L. Pras-Raves, Frédéric M. Vaz, Stephan Michel, Riekelt H. Houtkooper, Michel van Weeghel

Research output: Contribution to JournalArticleAcademicpeer-review

3 Citations (Scopus)


Lipid metabolism is under the control of the circadian system and circadian dysregulation has been linked to obesity and dyslipidemia. These factors and outcomes have also been associated to, or affected by, the process of aging. Here, we investigated whether murine white (WAT) and brown (BAT) adipose tissue lipids exhibit rhythmicity and if this is affected by aging. To this end, we have measured the 24 h lipid profiles of WAT and BAT using a global lipidomics analysis of > 1100 lipids. We observed rhythmicity in nearly all lipid classes including glycerolipids, glycerophospholipids, sterol lipids and sphingolipids. Overall, ~ 22% of the analyzed lipids were considered rhythmic in WAT and BAT. Despite a general accumulation of lipids upon aging the fraction of oscillating lipids decreased in both tissues to 14% and 18%, respectively. Diurnal profiles of lipids in BAT appeared to depend on the lipid acyl chain length and this specific regulation was lost in aged mice. Our study revealed how aging affects the rhythmicity of lipid metabolism and could contribute to the quest for targets that improve diurnal lipid homeostasis to maintain cardiometabolic health during aging.
Original languageEnglish
Article number5932
Pages (from-to)5932
JournalScientific reports
Issue number1
Publication statusPublished - 1 Dec 2021


  • Adipose Tissue, Brown/metabolism
  • Adipose Tissue, White/metabolism
  • Age Factors
  • Aging/metabolism
  • Animals
  • Biomarkers
  • Chromatography, High Pressure Liquid
  • Computational Biology/methods
  • Lipid Metabolism
  • Lipidomics/methods
  • Male
  • Mass Spectrometry
  • Mice

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