TY - JOUR
T1 - Allele-specific expression of the IL-1 alpha gene in human CD4+ T cell clones
AU - Bayley, Jean-Pierre
AU - van Rietschoten, Johanna G. I.
AU - Bakker, Aleida M.
AU - van Baarsen, Lisa
AU - Kaijzel, Eric L.
AU - Wierenga, Eddy A.
AU - van der Pouw Kraan, Tineke C. T. M.
AU - Huizinga, Tom W. J.
AU - Verweij, Cornelis L.
PY - 2003
Y1 - 2003
N2 - A number of reports have described the monoallelic expression of murine cytokine genes. Here we describe the monoallelic expression of the human IL-1alpha gene in CD4+ T cells. Analysis of peripheral blood T cell clones derived from healthy individuals revealed that the IL-1alpha gene shows predominantly monoallelic expression. Monoallelic expression was observed in Th0, Th1, and Th2 cell clones. In addition, we demonstrate monoallelic expression in T cell clones from rheumatoid arthritis patients derived from synovial fluid of the knee joint, suggesting that the occurrence of this phenomenon is not different from that in clones derived from healthy individuals. The finding of monoallelic expression of a cytokine gene in human CD4+ T cell clones provides evidence for allele-specific silencing/activation as another layer of regulation of IL-1alpha gene expression
AB - A number of reports have described the monoallelic expression of murine cytokine genes. Here we describe the monoallelic expression of the human IL-1alpha gene in CD4+ T cells. Analysis of peripheral blood T cell clones derived from healthy individuals revealed that the IL-1alpha gene shows predominantly monoallelic expression. Monoallelic expression was observed in Th0, Th1, and Th2 cell clones. In addition, we demonstrate monoallelic expression in T cell clones from rheumatoid arthritis patients derived from synovial fluid of the knee joint, suggesting that the occurrence of this phenomenon is not different from that in clones derived from healthy individuals. The finding of monoallelic expression of a cytokine gene in human CD4+ T cell clones provides evidence for allele-specific silencing/activation as another layer of regulation of IL-1alpha gene expression
U2 - https://doi.org/10.4049/jimmunol.171.5.2349
DO - https://doi.org/10.4049/jimmunol.171.5.2349
M3 - Article
C2 - 12928381
SN - 0022-1767
VL - 171
SP - 2349
EP - 2353
JO - Journal of immunology (Baltimore, Md.
JF - Journal of immunology (Baltimore, Md.
IS - 5
ER -