TY - JOUR
T1 - Analysis of Synthetic Monodisperse Polysaccharides by Wide Mass Range Ultrahigh-Resolution MALDI Mass Spectrometry
AU - Nicolardi, Simone
AU - Joseph, A. Abragam
AU - Zhu, Qian
AU - Shen, Zhengnan
AU - Pardo-Vargas, Alonso
AU - Chiodo, Fabrizio
AU - Molinaro, Antonio
AU - Silipo, Alba
AU - van der Burgt, Yuri E. M.
AU - Yu, Biao
AU - Seeberger, Peter H.
AU - Wuhrer, Manfred
N1 - Funding Information: We gratefully acknowledge the generous financial support of the Max Planck Society. Publisher Copyright: © 2021 The Authors. Copyright: Copyright 2021 Elsevier B.V., All rights reserved.
PY - 2021/3/16
Y1 - 2021/3/16
N2 - Carbohydrates, such as oligo- and polysaccharides, are highly abundant biopolymers that are involved in numerous processes. The study of their structure and functions is commonly based on a material that is isolated from complex natural sources. However, a more precise analysis requires pure compounds with well-defined structures that can be obtained from chemical or enzymatic syntheses. Novel synthetic strategies have increased the accessibility of larger monodisperse polysaccharides, posing a challenge to the analytical methods used for their molecular characterization. Here, we present wide mass range ultrahigh-resolution matrix-assisted laser desorption/ionization (MALDI) Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry (MS) as a powerful platform for the analysis of synthetic oligo- and polysaccharides. Synthetic carbohydrates 16-, 64-, 100-, and 151-mers were mass analyzed and characterized by MALDI in-source decay FT-ICR MS. Detection of fragment ions generated from glycosidic bond cleavage (or cross-ring cleavage) provided information of the monosaccharide content and the linkage type, allowing for the corroboration of the carbohydrate compositions and structures.
AB - Carbohydrates, such as oligo- and polysaccharides, are highly abundant biopolymers that are involved in numerous processes. The study of their structure and functions is commonly based on a material that is isolated from complex natural sources. However, a more precise analysis requires pure compounds with well-defined structures that can be obtained from chemical or enzymatic syntheses. Novel synthetic strategies have increased the accessibility of larger monodisperse polysaccharides, posing a challenge to the analytical methods used for their molecular characterization. Here, we present wide mass range ultrahigh-resolution matrix-assisted laser desorption/ionization (MALDI) Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry (MS) as a powerful platform for the analysis of synthetic oligo- and polysaccharides. Synthetic carbohydrates 16-, 64-, 100-, and 151-mers were mass analyzed and characterized by MALDI in-source decay FT-ICR MS. Detection of fragment ions generated from glycosidic bond cleavage (or cross-ring cleavage) provided information of the monosaccharide content and the linkage type, allowing for the corroboration of the carbohydrate compositions and structures.
UR - http://www.scopus.com/inward/record.url?scp=85103446544&partnerID=8YFLogxK
U2 - https://doi.org/10.1021/acs.analchem.1c00239
DO - https://doi.org/10.1021/acs.analchem.1c00239
M3 - Article
C2 - 33667082
SN - 0003-2700
VL - 93
SP - 4666
EP - 4675
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 10
ER -