TY - JOUR
T1 - Analyzing type 2 diabetes associations with the gut microbiome in individuals from two ethnic backgrounds living in the same geographic area
AU - Balvers, Manon
AU - Deschasaux, M. lanie
AU - van den Born, Bert-Jan
AU - Zwinderman, Koos
AU - Nieuwdorp, Max
AU - Levin, Evgeni
N1 - Funding Information: Funding: M.B. is appointed on a NNF CAMIT grant 2018 (to M.N.). M.N. is supported by a personal ZONMW VICI grant 2020 [09150182010020]. This research has been also supported by ZonMw for Technology Hotel grant [40–43500–98–5007]. The HELIUS study is conducted by the Amsterdam University Medical Centers, location AMC and the Public Health Service of Amsterdam. Both organizations provided core support for HELIUS. The HELIUS study is also funded by the Dutch Heart Foundation, the Netherlands Organization for Health Research and Development (ZonMw), the European Union (FP-7), and the European Fund for the Integration of non-EU immigrants (EIF). The study reported here was additionally supported by (an) additional grant(s) from the Dutch Heart Foundation [2010T084], ZonMw [200500003], European Union (FP-7) [278901], and European Fund for the Integration of non-EU immigrants (EIF) [2013EIF013] all assigned to K. Stronks. Funding Information: M.B. is appointed on a NNF CAMIT grant 2018 (to M.N.). M.N. is supported by a personal ZONMW VICI grant 2020 [09150182010020]. This research has been also supported by ZonMw for Technology Hotel grant [40?43500?98?5007]. The HELIUS study is conducted by the Amsterdam University Medical Centers, location AMC and the Public Health Service of Amsterdam. Both organizations provided core support for HELIUS. The HELIUS study is also funded by the Dutch Heart Foundation, the Netherlands Organization for Health Research and Development (ZonMw), the European Union (FP-7), and the European Fund for the Integration of non-EU immigrants (EIF). The study reported here was additionally supported by (an) additional grant(s) from the Dutch Heart Foundation [2010T084], ZonMw [200500003], European Union (FP-7) [278901], and European Fund for the Integration of non-EU immigrants (EIF) [2013EIF013] all assigned to K. Stronks. Acknowledgments: We are most grateful to the participants of the HELIUS study and the management team, research nurses, interviewers, research assistants and other staff who have taken part in gathering the data of this study. Some illustrations in the graphical abstract are from Servier Medical Art (smart.servier.com). Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2021/9/1
Y1 - 2021/9/1
N2 - It is currently unknown whether associations between gut microbiota composition and type 2 diabetes (T2D) differ according to the ethnic background of individuals. Thus, we studied these associations in participants from two ethnicities characterized by a high T2D prevalence and living in the same geographical area, using the Healthy Life In Urban Settings (HELIUS) study. We included 111 and 128 T2D participants on metformin (Met-T2D), 78 and 49 treatment-naïve T2D (TN-T2D) participants, as well as a 1:1 matched group of healthy controls from, respectively, African Surinamese and South-Asian Surinamese descent. Fecal microbiome profiles were obtained through 16S rRNA gene sequencing. Univariate and machine learning analyses were used to ex-plore the associations between T2D and the composition and function of the gut microbiome in both ethnicities, comparing Met-T2D and TN-T2D participants to their respective healthy control. We found a lower α-diversity for South-Asian Surinamese TN-T2D participants but no significant associations between TN-T2D status and the abundance of bacterial taxa or functional pathways. In African Surinamese participants, we did not find any association between TN-T2D status and the gut microbiome. With respect to Met-T2D participants, we identified several bacterial taxa and functional pathways with a significantly altered abundance in both ethnicities. More alterations were observed in South-Asian Surinamese. Some altered taxa and pathways observed in both ethnicities were previously related to metformin use. This included a strong negative association between the abundance of Romboutsia and Met-T2D status. Other bacterial taxa were consistent with previous observations in T2D, including reduced butyrate producers such as Anaerostipes hadrus. Hence, our results highlighted both shared and unique gut microbial biomarkers of Met-T2D in individuals from different ethnicities but living in the same geographical area. Future research using higher-resolution shotgun sequencing is needed to clarify the role of ethnicity in the association between T2D and gut microbiota composition.
AB - It is currently unknown whether associations between gut microbiota composition and type 2 diabetes (T2D) differ according to the ethnic background of individuals. Thus, we studied these associations in participants from two ethnicities characterized by a high T2D prevalence and living in the same geographical area, using the Healthy Life In Urban Settings (HELIUS) study. We included 111 and 128 T2D participants on metformin (Met-T2D), 78 and 49 treatment-naïve T2D (TN-T2D) participants, as well as a 1:1 matched group of healthy controls from, respectively, African Surinamese and South-Asian Surinamese descent. Fecal microbiome profiles were obtained through 16S rRNA gene sequencing. Univariate and machine learning analyses were used to ex-plore the associations between T2D and the composition and function of the gut microbiome in both ethnicities, comparing Met-T2D and TN-T2D participants to their respective healthy control. We found a lower α-diversity for South-Asian Surinamese TN-T2D participants but no significant associations between TN-T2D status and the abundance of bacterial taxa or functional pathways. In African Surinamese participants, we did not find any association between TN-T2D status and the gut microbiome. With respect to Met-T2D participants, we identified several bacterial taxa and functional pathways with a significantly altered abundance in both ethnicities. More alterations were observed in South-Asian Surinamese. Some altered taxa and pathways observed in both ethnicities were previously related to metformin use. This included a strong negative association between the abundance of Romboutsia and Met-T2D status. Other bacterial taxa were consistent with previous observations in T2D, including reduced butyrate producers such as Anaerostipes hadrus. Hence, our results highlighted both shared and unique gut microbial biomarkers of Met-T2D in individuals from different ethnicities but living in the same geographical area. Future research using higher-resolution shotgun sequencing is needed to clarify the role of ethnicity in the association between T2D and gut microbiota composition.
KW - Ethnicity
KW - Gut microbiome
KW - HELIUS study
KW - Metformin
KW - Treatment-naïve
KW - Type 2 diabetes
UR - http://www.scopus.com/inward/record.url?scp=85115139077&partnerID=8YFLogxK
U2 - https://doi.org/10.3390/nu13093289
DO - https://doi.org/10.3390/nu13093289
M3 - Article
C2 - 34579166
SN - 2072-6643
VL - 13
JO - NUTRIENTS
JF - NUTRIENTS
IS - 9
M1 - 3289
ER -