TY - JOUR
T1 - Antibiotic susceptibility profile of bacterial isolates from febrile children under 5 years of age in Nanoro, Burkina Faso
AU - Bonko, Massa dit Achille
AU - Tahita, Marc Christian
AU - Kiemde, Francois
AU - Lompo, Palpouguini
AU - Yougbaré, Sibidou
AU - Some, Athanase M.
AU - Tinto, Halidou
AU - Mens, Petra F.
AU - Menting, Sandra
AU - Schallig, Henk D. F. H.
N1 - Funding Information: The research was financially supported by a grant from the Netherlands Organization for Health Research and Development (ZonMw), project 205300005; RAPDIF: A Rapid Diagnostic test for undifferentiated Fevers and a Discovery Award granted to the research team by the NESTA Foundation (London, UK). Funding Information: The research was financially supported by a grant from the Netherlands Organization for Health Research and Development (ZonMw), project 205300005; RAPDIF: A Rapid Diagnostic test for undifferentiated Fevers and a Discovery Award granted to the research team by the NESTA Foundation (London, UK). We would like to acknowledge the study staff of the rural health facilities and the hospital CMA Saint Camille de Nanoro for their precious assistance to the work. We are very grateful to all the patients from whom the clinical isolates were obtained. We acknowledge the staff of the Microbiology Department of Clinical Research Unit of Nanoro (Burkina Faso) for their enormous help in performing this study. The American Type Culture Collection (ATCC??) provided standard reference strains Escherichia coli ATCC? 25922?, Salmonella thyphimurium ATCC? 14028?, Staphylococcus aureus ATCC? 25923?, Staphylococcus epidermidis ATCC? 14990?, Streptococcus pyogenes ATCC? 19615?, Enterococcus faecalis ATCC? 29212? and Streptococcus pneumonia ATCC? 49619? to the CRUN laboratory. Publisher Copyright: © 2021 The Authors Tropical Medicine & International Health Published by John Wiley & Sons Ltd Copyright: Copyright 2021 Elsevier B.V., All rights reserved.
PY - 2021/10
Y1 - 2021/10
N2 - Objectives: Antibiotics efficacy is severely threatened due to emerging resistance worldwide, but there is a paucity of antibiotics efficacy data for the West African region in general. Therefore, this study aimed to determine the antibiotic susceptibility profile of bacterial isolated from febrile children under 5 years of age in Nanoro (Burkina Faso). Methods: Blood, stool and urine samples were collected from 1099 febrile children attending peripheral health facilities and the referral hospital in Nanoro Health district. Bacterial isolates from these samples were assessed for their susceptibility against commonly used antibiotics by Kirby–Bauer method. Results: In total, 141 bacterial isolates were recovered from 127 febrile children of which 65 from blood, 65 from stool and 11 from urine. Salmonella isolates were most frequently isolated and found to be highly resistant to ampicillin (70%; 56/80) and trimethoprim–sulphamethoxazole (65%; 52/80). Escherichia coli isolates showed a high resistance rate to trimethoprim–sulphamethoxazole (100%), ampicillin (100%), ciprofloxacin (71.4%; 10/14), amoxicillin–clavulanate (64.3%; 9/14), ceftriaxone (64.3%; 9/14) and gentamycin (50%; 7/14). Moreover, half of the E. coli isolates produced ß-lactamase suggesting multi-drug resistance against β-lactam as well as non-β-lactam antibiotics. Multi-drug resistance was observed in 54.6% (59/108) of the isolates, mainly Gram-negative bacteria. Conclusions: This study showed high resistance rates to common antibiotics used to treat bacterial infections in Nanoro. The work prompts the need to expand antibiotic resistance surveillance studies in Burkina Faso.
AB - Objectives: Antibiotics efficacy is severely threatened due to emerging resistance worldwide, but there is a paucity of antibiotics efficacy data for the West African region in general. Therefore, this study aimed to determine the antibiotic susceptibility profile of bacterial isolated from febrile children under 5 years of age in Nanoro (Burkina Faso). Methods: Blood, stool and urine samples were collected from 1099 febrile children attending peripheral health facilities and the referral hospital in Nanoro Health district. Bacterial isolates from these samples were assessed for their susceptibility against commonly used antibiotics by Kirby–Bauer method. Results: In total, 141 bacterial isolates were recovered from 127 febrile children of which 65 from blood, 65 from stool and 11 from urine. Salmonella isolates were most frequently isolated and found to be highly resistant to ampicillin (70%; 56/80) and trimethoprim–sulphamethoxazole (65%; 52/80). Escherichia coli isolates showed a high resistance rate to trimethoprim–sulphamethoxazole (100%), ampicillin (100%), ciprofloxacin (71.4%; 10/14), amoxicillin–clavulanate (64.3%; 9/14), ceftriaxone (64.3%; 9/14) and gentamycin (50%; 7/14). Moreover, half of the E. coli isolates produced ß-lactamase suggesting multi-drug resistance against β-lactam as well as non-β-lactam antibiotics. Multi-drug resistance was observed in 54.6% (59/108) of the isolates, mainly Gram-negative bacteria. Conclusions: This study showed high resistance rates to common antibiotics used to treat bacterial infections in Nanoro. The work prompts the need to expand antibiotic resistance surveillance studies in Burkina Faso.
KW - antibiotic resistance
KW - bacteria
KW - febrile children
UR - http://www.scopus.com/inward/record.url?scp=85110976687&partnerID=8YFLogxK
U2 - https://doi.org/10.1111/tmi.13644
DO - https://doi.org/10.1111/tmi.13644
M3 - Article
C2 - 34185935
SN - 1360-2276
VL - 26
SP - 1220
EP - 1230
JO - Tropical Medicine & International Health
JF - Tropical Medicine & International Health
IS - 10
ER -