TY - JOUR
T1 - Applicability of quantitative optical imaging techniques for intraoperative perfusion diagnostics
T2 - A comparison of laser speckle contrast imaging, sidestream dark-field microscopy, and optical coherence tomography
AU - Jansen, S.M.
AU - de Bruin, D.M.
AU - Faber, D.J.
AU - Dobbe, I.J.G.G.
AU - Heeg, E.
AU - Milstein, D.M.J.
AU - Strackee, S.D.
AU - van Leeuwen, T.G.
PY - 2017/8
Y1 - 2017/8
N2 - Patient morbidity and mortality due to hemodynamic complications are a major problem in surgery. Optical techniques can image blood flow in real-time and high-resolution, thereby enabling perfusion monitoring intraoperatively. We tested the feasibility and validity of laser speckle contrast imaging (LSCI), optical coherence tomography (OCT), and sidestream dark-field microscopy (SDF) for perfusion diagnostics in a phantom model using whole blood. Microvessels with diameters of 50, 100, and 400μm were constructed in a scattering phantom. Perfusion was simulated by pumping heparinized human whole blood at five velocities (0 to 20mm/s). Vessel diameter and blood flow velocity were assessed with LSCI, OCT, and SDF. Quantification of vessel diameter was feasible with OCT and SDF. LSCI could only visualize the 400-μm vessel, perfusion units scaled nonlinearly with blood velocity. OCT could assess blood flow velocity in terms of inverse OCT speckle decorrelation time. SDF was not feasible to measure blood flow; however, for diluted blood the measurements were linear with the input velocity up to 1mm/s. LSCI, OCT, and SDF were feasible to visualize blood flow. Validated blood flow velocity measurements intraoperatively in the desired parameter (mL·min-1·g-1) remain challenging.
AB - Patient morbidity and mortality due to hemodynamic complications are a major problem in surgery. Optical techniques can image blood flow in real-time and high-resolution, thereby enabling perfusion monitoring intraoperatively. We tested the feasibility and validity of laser speckle contrast imaging (LSCI), optical coherence tomography (OCT), and sidestream dark-field microscopy (SDF) for perfusion diagnostics in a phantom model using whole blood. Microvessels with diameters of 50, 100, and 400μm were constructed in a scattering phantom. Perfusion was simulated by pumping heparinized human whole blood at five velocities (0 to 20mm/s). Vessel diameter and blood flow velocity were assessed with LSCI, OCT, and SDF. Quantification of vessel diameter was feasible with OCT and SDF. LSCI could only visualize the 400-μm vessel, perfusion units scaled nonlinearly with blood velocity. OCT could assess blood flow velocity in terms of inverse OCT speckle decorrelation time. SDF was not feasible to measure blood flow; however, for diluted blood the measurements were linear with the input velocity up to 1mm/s. LSCI, OCT, and SDF were feasible to visualize blood flow. Validated blood flow velocity measurements intraoperatively in the desired parameter (mL·min-1·g-1) remain challenging.
KW - biomedical optical imaging
KW - laser speckle contrast imaging
KW - medical diagnostics imaging
KW - optical coherence tomography
KW - perfusion
KW - phantom
KW - sidestream dark-field microscopy
UR - http://www.scopus.com/inward/record.url?scp=85028522255&partnerID=8YFLogxK
U2 - https://doi.org/10.1117/1.JBO.22.8.086004
DO - https://doi.org/10.1117/1.JBO.22.8.086004
M3 - Article
C2 - 28822141
SN - 1083-3668
VL - 22
SP - 86004
EP - 86009
JO - Journal of biomedical optics
JF - Journal of biomedical optics
IS - 8
M1 - 086004
ER -