Association of the thyroid stimulating hormone receptor gene (TSHR) with Graves' disease

Oliver J. Brand, Jeffrey C. Barrett, Matthew J. Simmonds, Paul R. Newby, Christopher J. McCabe, Christopher K. Bruce, Boris Kysela, Jackie D. Carr-Smith, Thomas Brix, Penny J. Hunt, Wilmar M. Wiersinga, Laszlo Hegedus, John Connell, John A. H. Wass, Jayne A. Franklyn, Anthony P. Weetman, Joanne M. Heward, Stephen C. L. Gough

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Abstract

Graves' disease (GD) is a common autoimmune disease (AID) that shares many of its susceptibility loci with other AIDs. The thyroid stimulating hormone receptor (TSHR) represents the primary autoantigen in GD, in which autoantibodies bind to the receptor and mimic its ligand, thyroid stimulating hormone (TSH), causing the characteristic clinical phenotype. Although early studies investigating the TSHR and GD proved inconclusive, more recently we provided convincing evidence for association of the TSHR region with disease. In the current study we investigated a combined panel of 98 SNPs, including 70 tag SNPs, across an extended 800Kb region of the TSHR to refine association in a cohort of 768 GD subjects and 768 matched controls. In total 28 SNPs revealed association with GD (P <0.05), with strongest SNP associations at rs179247 (chi(2)=32.45, P=8.90x10(-8), OR=1.53, 95% CI=1.32 - 1.78) and rs12101255 (chi(2)=30.91, P=1.95x10(-7), OR=1.55, 95% CI=1.33 - 1.81), both located in intron 1 of the TSHR. Association of the most associated SNP, rs179247, was replicated in 303 GD families (P=7.8x10(-4)). In addition we provide preliminary evidence that the disease-associated genotypes of rs179247 (AA) and rs12101255 (TT) show reduced mRNA expression ratios of flTSHR relative to two alternate TSHR mRNA splice variants
Original languageEnglish
Pages (from-to)1704-1713
JournalHuman Molecular Genetics
Volume18
Issue number9
DOIs
Publication statusPublished - 2009

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