Author Correction: Tissue patrol by resident memory CD8+ T cells in human skin (Nature Immunology, (2019), 20, 6, (756-764), 10.1038/s41590-019-0404-3)

Feline E. Dijkgraaf, Tiago R. Matos, Mark Hoogenboezem, Mireille Toebes, David W. Vredevoogd, Marjolijn Mertz, Bram van den Broek, Ji-Ying Song, Marcel B. M. Teunissen, Rosalie M. Luiten, Joost B. Beltman, Ton N. Schumacher

Research output: Contribution to journalComment/Letter to the editorAcademic

1 Citation (Scopus)

Abstract

In the version of this article initially published, the molecular-weight cutoff of the filtering unit was incorrectly given as 10,000 kDa; the correct value is 10 kDa. The incorrect value was given in Methods section “Generation of fluorescently labeled nanobodies,” in the sentences beginning “Purity of recombinant nanobody was assessed by SDS–PAGE ...” and “Subsequently, the unbound fraction was added ....” In the same section, the buffer composition for the hepta-mutant sortase incorrectly included 10 mM CaCl 2. The correct text is “To this end, purified GGGC–AF594 (80 μM) was incubated with purified nanobody–LPETGG-6×His (5 μM) and penta-mutant (5 M) or hepta-mutant (7 M) sortase (0.8 μM) for 2 h at 4 °C in 50 mM Tris pH 8 and 150 mM NaCl, and in the case of the penta-mutant, 10 mM CaCl 2 was added (sortase was produced in house according to a previously described protocol using sonification instead of French press 40). The errors have been corrected in the HTML and PDF versions of the article.

Original languageEnglish
Pages (from-to)696
Number of pages1
JournalNature immunology
Volume21
Issue number6
DOIs
Publication statusPublished - 1 Jun 2020

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