Peroxisomal homeostasis of metabolites and cofactors required for lipid metabolism

The work presented in this thesis relates to two biochemical pathways: fatty acid beta-oxidation and de novo ether lipid synthesis. Enzymes involved in these pathways are localized in a single-membrane-bounded organelle called peroxisome. The peroxisomal membrane forms an impermeable barrier for most of the precursors and products of the biochemical pathways, and thus they have to be transported by specialized membrane proteins or converted to smaller metabolites inside the peroxisomal lumen.
This thesis describes how certain metabolites, NAD(H) and CoA esters of fatty acids, are transported through the peroxisomal membrane and how a dysfunction of the transport affects peroxisomal metabolism.