TY - JOUR
T1 - Blood-borne circadian signal stimulates daily oscillations in actin dynamics and SRF activity
AU - Gerber, Alan
AU - Esnault, Cyril
AU - Aubert, Gregory
AU - Treisman, Richard
AU - Pralong, François
AU - Schibler, Ueli
N1 - Funding Information: We would like to thank Pascal Gos for transcardial perfusion, Jean-Marc Matter for his help with tissue preparation, Nicolas Roggli for the artwork, and David Suter (Harvard University, Boston) for critical comments on the manuscript. Work in the laboratory of U.S. was supported by the Swiss National Science Foundation (SNF 31-113565, SNF 31-128656/1 and the NCCR program grant Frontiers in Genetics), the European Research Council (ERC-AdG 250117), the State of Geneva, and the Louis Jeantet Foundation of Medicine. C.E. was funded in part by a long-term fellowship from EMBO. Work in the laboratory of R.T. was supported by core funding from Cancer Research UK and the European Research Council (ERC-Ad-G 268690). Work in the laboratory of F.P. was supported by the Swiss National Science Foundation (SNF grants 310000-122094 and 320030-124886). Copyright: Copyright 2013 Elsevier B.V., All rights reserved.
PY - 2013/1/31
Y1 - 2013/1/31
N2 - In peripheral tissues circadian gene expression can be driven either by local oscillators or by cyclic systemic cues controlled by the master clock in the brain's suprachiasmatic nucleus. In the latter case, systemic signals can activate immediate early transcription factors (IETFs) and thereby control rhythmic transcription. In order to identify IETFs induced by diurnal blood-borne signals, we developed an unbiased experimental strategy, dubbed Synthetic TAndem Repeat PROMoter (STAR-PROM) screening. This technique relies on the observation that most transcription factor binding sites exist at a relatively high frequency in random DNA sequences. Using STAR-PROM we identified serum response factor (SRF) as an IETF responding to oscillating signaling proteins present in human and rodent sera. Our data suggest that in mouse liver SRF is regulated via dramatic diurnal changes of actin dynamics, leading to the rhythmic translocation of the SRF coactivator Myocardin-related transcription factor-B (MRTF-B) into the nucleus.
AB - In peripheral tissues circadian gene expression can be driven either by local oscillators or by cyclic systemic cues controlled by the master clock in the brain's suprachiasmatic nucleus. In the latter case, systemic signals can activate immediate early transcription factors (IETFs) and thereby control rhythmic transcription. In order to identify IETFs induced by diurnal blood-borne signals, we developed an unbiased experimental strategy, dubbed Synthetic TAndem Repeat PROMoter (STAR-PROM) screening. This technique relies on the observation that most transcription factor binding sites exist at a relatively high frequency in random DNA sequences. Using STAR-PROM we identified serum response factor (SRF) as an IETF responding to oscillating signaling proteins present in human and rodent sera. Our data suggest that in mouse liver SRF is regulated via dramatic diurnal changes of actin dynamics, leading to the rhythmic translocation of the SRF coactivator Myocardin-related transcription factor-B (MRTF-B) into the nucleus.
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U2 - https://doi.org/10.1016/j.cell.2012.12.027
DO - https://doi.org/10.1016/j.cell.2012.12.027
M3 - Article
C2 - 23374345
SN - 0092-8674
VL - 152
SP - 492
EP - 503
JO - Cell
JF - Cell
IS - 3
ER -
