TY - GEN
T1 - Bringing third and second harmonic generation microscopy into the clinic for the assessment of fresh lung tissue
AU - Van Huizen, Laura M.G.
AU - Seinstra, Daniëlle
AU - Dickhoff, Chris
AU - Radonic, Teodora
AU - Bahce, Idris
AU - Van Mourik, Frank
AU - Annema, Jouke T.
AU - Daniels, Johannes M.A.
AU - Van Boven, Wim Jan P.
AU - Groot, Marie Louise
PY - 2019/1/1
Y1 - 2019/1/1
N2 - Third and second harmonic generation (THG/SHG) microscopy is a novel imaging technique that has been suggested as a promising clinical tool, mainly for cancer diagnosis. Here, we test for the first time a compact, transportable ex-vivo THG/SHG microscope (Tritos Diagnostics B.V.) for clinical use. In lung surgery, immediate feedback on the nature of the excised tissue is important. We will present the first results of an ongoing study in which we image both lung tumor tissue immediately after excision and frozen section biopsies, and compare the images to the golden standard histology. Prior to this study, we performed a set of experiments collecting 3D images of mouse lung tissue to show that the combination of THG and SHG microscopy reveals key lung morphology components.
AB - Third and second harmonic generation (THG/SHG) microscopy is a novel imaging technique that has been suggested as a promising clinical tool, mainly for cancer diagnosis. Here, we test for the first time a compact, transportable ex-vivo THG/SHG microscope (Tritos Diagnostics B.V.) for clinical use. In lung surgery, immediate feedback on the nature of the excised tissue is important. We will present the first results of an ongoing study in which we image both lung tumor tissue immediately after excision and frozen section biopsies, and compare the images to the golden standard histology. Prior to this study, we performed a set of experiments collecting 3D images of mouse lung tissue to show that the combination of THG and SHG microscopy reveals key lung morphology components.
KW - Clinic
KW - Lung tissue
KW - Second harmonic generation microscopy
KW - Third harmonic generation microscopy
KW - Validation
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U2 - https://doi.org/10.1117/12.2527899
DO - https://doi.org/10.1117/12.2527899
M3 - Conference contribution
VL - 11079
T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
BT - Medical Laser Applications and Laser-Tissue Interactions IX
A2 - Lilge, Lothar D.
A2 - Philipp, Carsten M.
PB - SPIE
T2 - Medical Laser Applications and Laser-Tissue Interactions IX 2019
Y2 - 23 June 2019 through 24 June 2019
ER -