Bringing third and second harmonic generation microscopy into the clinic for the assessment of fresh lung tissue

Laura M.G. Van Huizen, Daniëlle Seinstra, Chris Dickhoff, Teodora Radonic, Idris Bahce, Frank Van Mourik, Jouke T. Annema, Johannes M.A. Daniels, Wim Jan P. Van Boven, Marie Louise Groot

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review


Third and second harmonic generation (THG/SHG) microscopy is a novel imaging technique that has been suggested as a promising clinical tool, mainly for cancer diagnosis. Here, we test for the first time a compact, transportable ex-vivo THG/SHG microscope (Tritos Diagnostics B.V.) for clinical use. In lung surgery, immediate feedback on the nature of the excised tissue is important. We will present the first results of an ongoing study in which we image both lung tumor tissue immediately after excision and frozen section biopsies, and compare the images to the golden standard histology. Prior to this study, we performed a set of experiments collecting 3D images of mouse lung tissue to show that the combination of THG and SHG microscopy reveals key lung morphology components.

Original languageEnglish
Title of host publicationMedical Laser Applications and Laser-Tissue Interactions IX
EditorsLothar D. Lilge, Carsten M. Philipp
Number of pages3
ISBN (Electronic)9781510628519, 9781557528209
Publication statusPublished - 1 Jan 2019
EventMedical Laser Applications and Laser-Tissue Interactions IX 2019 - Munich, Germany
Duration: 23 Jun 201924 Jun 2019

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE


ConferenceMedical Laser Applications and Laser-Tissue Interactions IX 2019


  • Clinic
  • Lung tissue
  • Second harmonic generation microscopy
  • Third harmonic generation microscopy
  • Validation

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