TY - JOUR
T1 - Can you trust your cryostat? Reproducibility of cryostat section thickness
AU - de Witt Hamer, Philip C.
AU - Bleeker, Fonnet E.
AU - Zwinderman, Aeilko H.
AU - van Noorden, Cornelis J. F.
PY - 2006
Y1 - 2006
N2 - Reproducibility of cryostat section thickness is required for valid quantitative microscopy. This is generally pursued by motorized sectioning using a low but constant speed. The purpose of our study was to compare variation in section thickness between motorized and manual cryostat sectioning. Serial sections were cut from a frozen block of homogenized tissue on different days. Lactate dehydrogenase activity was histochemically detected and calibrated absorbance measurements were taken. The coefficients of variation of measurements was 9.7% for motorized sectioning and 3.3% for manual sectioning. In conclusion, section thickness is similarly reproducible after manual sectioning compared with motorized sectioning, if not better
AB - Reproducibility of cryostat section thickness is required for valid quantitative microscopy. This is generally pursued by motorized sectioning using a low but constant speed. The purpose of our study was to compare variation in section thickness between motorized and manual cryostat sectioning. Serial sections were cut from a frozen block of homogenized tissue on different days. Lactate dehydrogenase activity was histochemically detected and calibrated absorbance measurements were taken. The coefficients of variation of measurements was 9.7% for motorized sectioning and 3.3% for manual sectioning. In conclusion, section thickness is similarly reproducible after manual sectioning compared with motorized sectioning, if not better
U2 - https://doi.org/10.1002/jemt.20354
DO - https://doi.org/10.1002/jemt.20354
M3 - Article
C2 - 16972226
SN - 1059-910X
VL - 69
SP - 835
EP - 838
JO - MICROSCOPY RESEARCH AND TECHNIQUE
JF - MICROSCOPY RESEARCH AND TECHNIQUE
IS - 10
ER -