TY - JOUR
T1 - Cardiac expression of Gal4 causes cardiomyopathy in a dose-dependent manner
AU - Habets, Petra E. M. H.
AU - Clout, Danielle E. W.
AU - Lekanne Deprez, Ronald H.
AU - van Roon, Marian A.
AU - Moorman, Antoon F. M.
AU - Christoffels, Vincent M.
PY - 2003
Y1 - 2003
N2 - Cardiac expression of a transgene is a common approach for determining the role of gene products in the processes underlying cardiomyopathy and heart failure (HF). We have generated transgenic mice that express the 'harmless' yeast transcription factor Gal4 in the heart under control of the alpha-myosin heavy chain promoter and found that expression of this gene causes cardiomyopathy and HF, the severity of which correlated with the number of copies of the transgene integrated into the genome and with the expression level. A line with a single copy of the transgene targeted to the hprt locus correctly expressed the transgene but did not develop cardiomyopathy. Our results indicate that expression of a transgene in the heart may non-specifically cause HF in a dose-dependent manner
AB - Cardiac expression of a transgene is a common approach for determining the role of gene products in the processes underlying cardiomyopathy and heart failure (HF). We have generated transgenic mice that express the 'harmless' yeast transcription factor Gal4 in the heart under control of the alpha-myosin heavy chain promoter and found that expression of this gene causes cardiomyopathy and HF, the severity of which correlated with the number of copies of the transgene integrated into the genome and with the expression level. A line with a single copy of the transgene targeted to the hprt locus correctly expressed the transgene but did not develop cardiomyopathy. Our results indicate that expression of a transgene in the heart may non-specifically cause HF in a dose-dependent manner
U2 - https://doi.org/10.1023/A:1026055612227
DO - https://doi.org/10.1023/A:1026055612227
M3 - Article
C2 - 14609031
SN - 0142-4319
VL - 24
SP - 205
EP - 209
JO - Journal of muscle research and cell motility
JF - Journal of muscle research and cell motility
IS - 2-3
ER -