TY - JOUR
T1 - CD45RB Glycosylation and Ig Isotype Define Maturation of Functionally Distinct B Cell Subsets in Human Peripheral Blood
AU - Koers, Jana
AU - Pollastro, Sabrina
AU - Tol, Simon
AU - Pico-Knijnenburg, Ingrid
AU - Derksen, Ninotska I. L.
AU - van Schouwenburg, Pauline A.
AU - van der Burg, Mirjam
AU - van Ham, S. Marieke
AU - Rispens, Theo
N1 - Funding Information: This study was supported by Landsteiner Foundation for Blood Transfusion Research (Grant1626). Publisher Copyright: Copyright © 2022 Koers, Pollastro, Tol, Pico-Knijnenburg, Derksen, van Schouwenburg, van der Burg, van Ham and Rispens.
PY - 2022/4/28
Y1 - 2022/4/28
N2 - Glycosylation of CD45RB (RB+) has recently been identified to mark antigen-experienced B cells, independent of their CD27 expression. By using a novel combination of markers including CD45RB glycosylation, CD27 and IgM/IgD isotype expression we segregated human peripheral blood B cell subsets and investigated their IGHV repertoire and in vitro functionality. We observed distinct maturation stages for CD27-RB+ cells, defined by differential expression of non-switched Ig isotypes. CD27-RB+ cells, which only express IgM, were more matured in terms of Ig gene mutation levels and function as compared to CD27-RB+ cells that express both IgM and IgD or cells that were CD27-RB-. Moreover, CD27-RB+IgM+ cells already showed remarkable rigidity in IgM isotype commitment, different from CD27-RB+IgMD+ and CD27-RB- cells that still demonstrated great plasticity in B cell fate decision. Thus, glycosylation of CD45RB is indicative for antigen-primed B cells, which are, dependent on the Ig isotype, functionally distinct.
AB - Glycosylation of CD45RB (RB+) has recently been identified to mark antigen-experienced B cells, independent of their CD27 expression. By using a novel combination of markers including CD45RB glycosylation, CD27 and IgM/IgD isotype expression we segregated human peripheral blood B cell subsets and investigated their IGHV repertoire and in vitro functionality. We observed distinct maturation stages for CD27-RB+ cells, defined by differential expression of non-switched Ig isotypes. CD27-RB+ cells, which only express IgM, were more matured in terms of Ig gene mutation levels and function as compared to CD27-RB+ cells that express both IgM and IgD or cells that were CD27-RB-. Moreover, CD27-RB+IgM+ cells already showed remarkable rigidity in IgM isotype commitment, different from CD27-RB+IgMD+ and CD27-RB- cells that still demonstrated great plasticity in B cell fate decision. Thus, glycosylation of CD45RB is indicative for antigen-primed B cells, which are, dependent on the Ig isotype, functionally distinct.
KW - B cell subset heterogeneity
KW - B-Lymphocyte Subsets/metabolism
KW - CD27
KW - CD45RB glycosylation
KW - Glycosylation
KW - Humans
KW - Immunoglobulin D/metabolism
KW - Immunoglobulin Isotypes/metabolism
KW - Immunoglobulin M/metabolism
KW - Leukocyte Common Antigens/immunology
KW - Tumor Necrosis Factor Receptor Superfamily, Member 7/metabolism
KW - memory B cells
KW - naive B cells
UR - http://www.scopus.com/inward/record.url?scp=85130029998&partnerID=8YFLogxK
UR - https://pure.uva.nl/ws/files/122046991/Table_1.XLSX
UR - https://pure.uva.nl/ws/files/122046993/Supplementary_Figures_CD45RB_Glycosylation.pdf
U2 - https://doi.org/10.3389/fimmu.2022.891316
DO - https://doi.org/10.3389/fimmu.2022.891316
M3 - Article
C2 - 35572548
SN - 1664-3224
VL - 13
JO - Frontiers in immunology
JF - Frontiers in immunology
M1 - 891316
ER -