TY - JOUR
T1 - CD62L Is a Functional and Phenotypic Marker for Circulating Innate Lymphoid Cell Precursors
AU - Bar-Ephraim, Yotam E.
AU - Koning, Jasper J.
AU - Burniol Ruiz, Estefany
AU - Konijn, Tanja
AU - Mourits, Vera P.
AU - Lakeman, Kim A.
AU - Boon, Louis
AU - Bögels, Marijn
AU - van Maanen, J. Peter
AU - Den Haan, Joke M. M.
AU - van Egmond, Marjolein
AU - Bouma, Gerd
AU - Reijmers, Rogier M.
AU - Mebius, Reina E.
PY - 2019/1/1
Y1 - 2019/1/1
N2 - Innate lymphoid cells (ILCs) guard epithelial tissue integrity during homeostasis, but can be potent immune effector cells during inflammation. Precursors to all ILC subsets (ILC precursors [ILCP]) have been identified in human peripheral blood (PB). We found that during homeostasis, ILCP in PB of mouse and human expressed homing receptors for secondary lymphoid organs, mainly CD62L. These ILCP entered mouse lymph nodes in a CD62L-dependent way and relied on S1P receptors for their exit. Importantly, CD62L expression was absent on human ILCs expressing NKp44 in tonsils and PB of Crohn disease patients, and relatively fewer CD62L+ ILCP were present in PB of Crohn disease patients. These data are in agreement with selective expression of CD62L on nonactivated ILCP. As such, we conclude that CD62L not only serves as a functional marker of ILCP, but has potential to be used in the clinic as a diagnostic marker in inflammatory disorders.
AB - Innate lymphoid cells (ILCs) guard epithelial tissue integrity during homeostasis, but can be potent immune effector cells during inflammation. Precursors to all ILC subsets (ILC precursors [ILCP]) have been identified in human peripheral blood (PB). We found that during homeostasis, ILCP in PB of mouse and human expressed homing receptors for secondary lymphoid organs, mainly CD62L. These ILCP entered mouse lymph nodes in a CD62L-dependent way and relied on S1P receptors for their exit. Importantly, CD62L expression was absent on human ILCs expressing NKp44 in tonsils and PB of Crohn disease patients, and relatively fewer CD62L+ ILCP were present in PB of Crohn disease patients. These data are in agreement with selective expression of CD62L on nonactivated ILCP. As such, we conclude that CD62L not only serves as a functional marker of ILCP, but has potential to be used in the clinic as a diagnostic marker in inflammatory disorders.
UR - https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85059230711&origin=inward
UR - https://www.ncbi.nlm.nih.gov/pubmed/30504420
U2 - https://doi.org/10.4049/jimmunol.1701153
DO - https://doi.org/10.4049/jimmunol.1701153
M3 - Article
C2 - 30504420
SN - 0022-1767
VL - 202
SP - 171
EP - 182
JO - Journal of Immunology
JF - Journal of Immunology
IS - 1
ER -