CD9 and ITGA3 are regulated during HIV-1 infection in macrophages to support viral replication

Zita Kruize; Viviana Cobos Jiménez; Fernando O. Martinez; Riccardo di Vincenzo; Karel A. van Dort; Ad C. van Nuenen; Thijs Booiman; Neeltje A. Kootstra

doi:https://doi.org/10.1016/j.virol.2021.07.002

CD9 and ITGA3 are regulated during HIV-1 infection in macrophages to support viral replication

Zita Kruize, Viviana Cobos Jiménez, Fernando O. Martinez, Riccardo di Vincenzo, Karel A. van Dort, Ad C. van Nuenen, Thijs Booiman, Neeltje A. Kootstra

Research output: Contribution to journal › Article › Academic › peer-review

4 Citations (Scopus)

Abstract

Monocytes/macrophages are important target cells for HIV-1. Here, we investigated whether HIV-1 induces changes in the macrophage gene expression profile to support viral replication. We observed that the macrophage gene expression profiles dramatically changed upon HIV-1 infection. The majority of the HIV-1 regulated genes were also differentially expressed in M2a macrophages. The biological functions associated with the HIV-1 induced gene expression profile in macrophages were mainly related to inflammatory responses. CD9 and ITGA3 were among the top genes upregulated upon HIV-1 infection. We showed that these genes support viral replication and that downregulation of these genes decreased HIV-1 replication in macrophages. Here we showed that HIV-1 infection of macrophages induces a gene expression profile that may dampen inflammatory responses. CD9 and ITGA3 were among the top genes regulated by HIV-1 and were shown to support viral production most likely at the level of viral budding and release.

Original language	English
Pages (from-to)	9-18
Number of pages	10
Journal	Virology
Volume	562
DOIs	https://doi.org/10.1016/j.virol.2021.07.002
Publication status	Published - 1 Oct 2021

Keywords

Assembly
Budding
CD9
HIV-1
ITGA3
Macrophages
Viral replication

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@article{4566ab0a100541c9af626f49f514bc33,

title = "CD9 and ITGA3 are regulated during HIV-1 infection in macrophages to support viral replication",

abstract = "Monocytes/macrophages are important target cells for HIV-1. Here, we investigated whether HIV-1 induces changes in the macrophage gene expression profile to support viral replication. We observed that the macrophage gene expression profiles dramatically changed upon HIV-1 infection. The majority of the HIV-1 regulated genes were also differentially expressed in M2a macrophages. The biological functions associated with the HIV-1 induced gene expression profile in macrophages were mainly related to inflammatory responses. CD9 and ITGA3 were among the top genes upregulated upon HIV-1 infection. We showed that these genes support viral replication and that downregulation of these genes decreased HIV-1 replication in macrophages. Here we showed that HIV-1 infection of macrophages induces a gene expression profile that may dampen inflammatory responses. CD9 and ITGA3 were among the top genes regulated by HIV-1 and were shown to support viral production most likely at the level of viral budding and release.",

keywords = "Assembly, Budding, CD9, HIV-1, ITGA3, Macrophages, Viral replication",

author = "Zita Kruize and {Cobos Jim{\'e}nez}, Viviana and Martinez, {Fernando O.} and {di Vincenzo}, Riccardo and {van Dort}, {Karel A.} and {van Nuenen}, {Ad C.} and Thijs Booiman and Kootstra, {Neeltje A.}",

note = "Funding Information: This work was supported by the Academic Medical Center of the University of Amsterdam, The Netherlands. Publisher Copyright: {\textcopyright} 2021 The Authors",

year = "2021",

month = oct,

day = "1",

doi = "https://doi.org/10.1016/j.virol.2021.07.002",

language = "English",

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T1 - CD9 and ITGA3 are regulated during HIV-1 infection in macrophages to support viral replication

AU - Kruize, Zita

AU - Cobos Jiménez, Viviana

AU - Martinez, Fernando O.

AU - di Vincenzo, Riccardo

AU - van Dort, Karel A.

AU - van Nuenen, Ad C.

AU - Booiman, Thijs

AU - Kootstra, Neeltje A.

PY - 2021/10/1

Y1 - 2021/10/1

N2 - Monocytes/macrophages are important target cells for HIV-1. Here, we investigated whether HIV-1 induces changes in the macrophage gene expression profile to support viral replication. We observed that the macrophage gene expression profiles dramatically changed upon HIV-1 infection. The majority of the HIV-1 regulated genes were also differentially expressed in M2a macrophages. The biological functions associated with the HIV-1 induced gene expression profile in macrophages were mainly related to inflammatory responses. CD9 and ITGA3 were among the top genes upregulated upon HIV-1 infection. We showed that these genes support viral replication and that downregulation of these genes decreased HIV-1 replication in macrophages. Here we showed that HIV-1 infection of macrophages induces a gene expression profile that may dampen inflammatory responses. CD9 and ITGA3 were among the top genes regulated by HIV-1 and were shown to support viral production most likely at the level of viral budding and release.

AB - Monocytes/macrophages are important target cells for HIV-1. Here, we investigated whether HIV-1 induces changes in the macrophage gene expression profile to support viral replication. We observed that the macrophage gene expression profiles dramatically changed upon HIV-1 infection. The majority of the HIV-1 regulated genes were also differentially expressed in M2a macrophages. The biological functions associated with the HIV-1 induced gene expression profile in macrophages were mainly related to inflammatory responses. CD9 and ITGA3 were among the top genes upregulated upon HIV-1 infection. We showed that these genes support viral replication and that downregulation of these genes decreased HIV-1 replication in macrophages. Here we showed that HIV-1 infection of macrophages induces a gene expression profile that may dampen inflammatory responses. CD9 and ITGA3 were among the top genes regulated by HIV-1 and were shown to support viral production most likely at the level of viral budding and release.

KW - Assembly

KW - Budding

KW - CD9

KW - HIV-1

KW - ITGA3

KW - Macrophages

KW - Viral replication

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U2 - https://doi.org/10.1016/j.virol.2021.07.002

DO - https://doi.org/10.1016/j.virol.2021.07.002

M3 - Article

C2 - 34242748

SN - 0042-6822

VL - 562

SP - 9

EP - 18

JO - Virology

JF - Virology

ER -

CD9 and ITGA3 are regulated during HIV-1 infection in macrophages to support viral replication

Abstract

Keywords

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