Abstract
BACKGROUND AND OBJECTIVES: Buffy coat-derived granulocytes have been described as an alternative to the apheresis product from donors pretreated with dexamethasone and granulocyte colony-stimulating factor (G-CSF). The latter is - dependent on the local and national settings - obtained following a demanding and time-consuming procedure, which is undesirable in critically ill septic patients. In contrast, buffy coat-derived products have a large volume and are often heavily contaminated with red cells and platelets. We developed a new pooled buffy coat-derived product with high purity and small volume, and performed a comprehensive functional characterization of these granulocytes.
MATERIALS AND METHODS: We pooled ten buffy coats following the production of platelet concentrates. Saline 0·9% was added to decrease the viscosity and the product was split into plasma, red cells and a 'super' buffy coat. Functional data of the granulocytes were compared to those obtained with granulocytes from healthy controls and G-CSF/dexamethasone-pretreated donors.
RESULTS: Buffy coat-derived granulocytes showed adhesion, chemotaxis, reactive oxygen species production, degranulation, NETosis and in vitro killing of Staphylococcus aureus, Escherichia coli and Aspergillus species comparable to control and G-CSF/dexamethasone-derived granulocytes. Candida killing was superior compared to G-CSF/dexamethasone-derived granulocytes. Immunophenotyping was normal; especially no signs of activation in the buffy coat-derived granulocytes were seen. Viability was reduced. Buffy coats are readily available in the regular blood production process and would take away the concerns around the apheresis product.
CONCLUSION: The product described appears a promising alternative for transfusion purposes.
Original language | English |
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Pages (from-to) | 173-182 |
Number of pages | 10 |
Journal | Vox sanguinis |
Volume | 112 |
Issue number | 2 |
Early online date | 2017 |
DOIs | |
Publication status | Published - Feb 2017 |
Keywords
- Adult
- Antigens, Surface
- Blood Buffy Coat
- Blood Component Removal
- Blood Donors
- Blood Platelets
- Cell Adhesion
- Cell Survival
- Chemotaxis
- Dexamethasone
- Granulocyte Colony-Stimulating Factor
- Granulocytes
- Humans
- Immunophenotyping
- Journal Article
- Leukocyte Count
- Male
- NADPH Oxidases
- Reactive Oxygen Species