TY - JOUR
T1 - Characterization of human phagocyte-derived chitotriosidase, a component of innate immunity
AU - van Eijk, Marco
AU - van Roomen, Cindy P. A. A.
AU - Renkema, G. Herma
AU - Bussink, Anton P.
AU - Andrews, Laura
AU - Blommaart, Edward F. C.
AU - Sugar, Alan
AU - Verhoeven, Arthur J.
AU - Boot, Rolf G.
AU - Aerts, Johannes M. F. G.
PY - 2005
Y1 - 2005
N2 - Man has been found to produce highly conserved chitinases. The most prominent is the phagocyte-derived chitotriosidase, the plasma levels of which are markedly elevated in some pathological conditions. Here, we report that both polymorphonuclear neutrophils (PMNs) and macrophages (m phi) are a source of chitotriosidase. The enzyme is located in specific granules of human PMNs and secreted following stimulation with granulocyte macrophage colony-stimulating factor (GM-CSF). In addition, GM-CSF induces expression of chitotriosidase in m phi that constitutively secrete the enzyme and partly accumulate it in their lysosomes. Studies with recombinant human chitotriosidase revealed that the enzyme targets chitin-containing fungi. These findings are consistent with earlier observations concerning anti-fungal activity of homologous plant chitinases and beneficial effects of GM-CSF administration in individuals suffering from invasive fungal infections. In conclusion, chitotriosidase should be viewed as a component of the innate immunity that may play a role in defence against chitin-containing pathogens and the expression and release of which by human phagocytes is highly regulated
AB - Man has been found to produce highly conserved chitinases. The most prominent is the phagocyte-derived chitotriosidase, the plasma levels of which are markedly elevated in some pathological conditions. Here, we report that both polymorphonuclear neutrophils (PMNs) and macrophages (m phi) are a source of chitotriosidase. The enzyme is located in specific granules of human PMNs and secreted following stimulation with granulocyte macrophage colony-stimulating factor (GM-CSF). In addition, GM-CSF induces expression of chitotriosidase in m phi that constitutively secrete the enzyme and partly accumulate it in their lysosomes. Studies with recombinant human chitotriosidase revealed that the enzyme targets chitin-containing fungi. These findings are consistent with earlier observations concerning anti-fungal activity of homologous plant chitinases and beneficial effects of GM-CSF administration in individuals suffering from invasive fungal infections. In conclusion, chitotriosidase should be viewed as a component of the innate immunity that may play a role in defence against chitin-containing pathogens and the expression and release of which by human phagocytes is highly regulated
U2 - https://doi.org/10.1093/intimm/dxh328
DO - https://doi.org/10.1093/intimm/dxh328
M3 - Article
C2 - 16214810
SN - 0953-8178
VL - 17
SP - 1505
EP - 1512
JO - International Immunology
JF - International Immunology
IS - 11
ER -