Chromatin Immunoprecipitation Sequencing (ChIP-seq) Protocol for Small Amounts of Frozen Biobanked Cardiac Tissue

Jiayi Pei; Noortje A M van den Dungen; Folkert W Asselbergs; Michal Mokry; Magdalena Harakalova

doi:https://doi.org/10.1007/978-1-0716-2140-0_6

Chromatin Immunoprecipitation Sequencing (ChIP-seq) Protocol for Small Amounts of Frozen Biobanked Cardiac Tissue

Jiayi Pei, Noortje A M van den Dungen, Folkert W Asselbergs, Michal Mokry, Magdalena Harakalova

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Chromatin immunoprecipitation and sequencing (ChIP-seq) is a well-established method to study the epigenetic profile at the genome-wide scale, including histone modifications and DNA-protein interactions. It provides valuable insights to better understand disease mechanisms. Here we present an optimized ChIP-seq protocol suitable for human cardiac tissues, especially the frozen biobanked small biopsy samples.

Original language	English
Pages (from-to)	97-111
Number of pages	15
Journal	Methods in molecular biology (Clifton, N.J.)
Volume	2458
DOIs	https://doi.org/10.1007/978-1-0716-2140-0_6
Publication status	Published - 2022
Externally published	Yes

Keywords

Antibody
Cardiac tissues
Chromatin immunoprecipitation
Enhancers
Promoters
Sequencing
Small biopsy

Access to Document

https://doi.org/10.1007/978-1-0716-2140-0_6

Cite this

@article{030452ce86f74427ae21ef1bbc2d21d1,

title = "Chromatin Immunoprecipitation Sequencing (ChIP-seq) Protocol for Small Amounts of Frozen Biobanked Cardiac Tissue",

abstract = "Chromatin immunoprecipitation and sequencing (ChIP-seq) is a well-established method to study the epigenetic profile at the genome-wide scale, including histone modifications and DNA-protein interactions. It provides valuable insights to better understand disease mechanisms. Here we present an optimized ChIP-seq protocol suitable for human cardiac tissues, especially the frozen biobanked small biopsy samples.",

keywords = "Antibody, Cardiac tissues, Chromatin immunoprecipitation, Enhancers, Promoters, Sequencing, Small biopsy",

author = "Jiayi Pei and {van den Dungen}, {Noortje A M} and Asselbergs, {Folkert W} and Michal Mokry and Magdalena Harakalova",

note = "Publisher Copyright: {\textcopyright} 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",

year = "2022",

doi = "https://doi.org/10.1007/978-1-0716-2140-0_6",

language = "English",

volume = "2458",

pages = "97--111",

journal = "Methods in molecular biology (Clifton, N.J.)",

issn = "1064-3745",

publisher = "Humana Press",

}

TY - JOUR

T1 - Chromatin Immunoprecipitation Sequencing (ChIP-seq) Protocol for Small Amounts of Frozen Biobanked Cardiac Tissue

AU - Pei, Jiayi

AU - van den Dungen, Noortje A M

AU - Asselbergs, Folkert W

AU - Mokry, Michal

AU - Harakalova, Magdalena

PY - 2022

Y1 - 2022

N2 - Chromatin immunoprecipitation and sequencing (ChIP-seq) is a well-established method to study the epigenetic profile at the genome-wide scale, including histone modifications and DNA-protein interactions. It provides valuable insights to better understand disease mechanisms. Here we present an optimized ChIP-seq protocol suitable for human cardiac tissues, especially the frozen biobanked small biopsy samples.

AB - Chromatin immunoprecipitation and sequencing (ChIP-seq) is a well-established method to study the epigenetic profile at the genome-wide scale, including histone modifications and DNA-protein interactions. It provides valuable insights to better understand disease mechanisms. Here we present an optimized ChIP-seq protocol suitable for human cardiac tissues, especially the frozen biobanked small biopsy samples.

KW - Antibody

KW - Cardiac tissues

KW - Chromatin immunoprecipitation

KW - Enhancers

KW - Promoters

KW - Sequencing

KW - Small biopsy

UR - http://www.scopus.com/inward/record.url?scp=85123963418&partnerID=8YFLogxK

U2 - https://doi.org/10.1007/978-1-0716-2140-0_6

DO - https://doi.org/10.1007/978-1-0716-2140-0_6

M3 - Article

C2 - 35103964

SN - 1064-3745

VL - 2458

SP - 97

EP - 111

JO - Methods in molecular biology (Clifton, N.J.)

JF - Methods in molecular biology (Clifton, N.J.)

ER -

Chromatin Immunoprecipitation Sequencing (ChIP-seq) Protocol for Small Amounts of Frozen Biobanked Cardiac Tissue

Abstract

Keywords

Access to Document

Other files and links

Cite this