TY - JOUR
T1 - Clinical applications of cell-free fetal DNA from maternal plasma
AU - Rijnders, Robbert J. P.
AU - Christiaens, Godelieve C. M. L.
AU - Bossers, Bernadette
AU - van der Smagt, Jasper J.
AU - van der Schoot, C. Ellen
AU - de Haas, Masja
PY - 2004
Y1 - 2004
N2 - OBJECTIVE: To describe our clinical experience with detection and analysis of cell-free fetal DNA derived from maternal plasma for prenatal sexing and fetal rhesus-D typing. METHODS: Real-time quantitative polymerase chain reactions (PCRs) of rhesus-D sequences and the SRY gene were validated and offered to patients with an enhanced risk for sex-linked fetal pathology and patients with rhesus-D antibodies. RESULTS: In die validation group, 72 samples were analyzed. Sensitivity of the rhesus-D real-time quantitative PCR in maternal plasma was 100% (95% confidence interval [CI]91.8%, 100%) and specificity was 96.6% (95% CI 82.2%,99.9%). Sensitivity of the SRY real-time quantitative PCR was 97.2% (95% CI 85.5%, 99.9%), and specificity was 100% (95% CI 88.1%, 100%). The technique was used successfully in a clinical setting in 24 women. Overall, invasive tests were avoided in 41.7% of these patients. CONCLUSION: Detection of cell-free fetal DNA from maternal plasma is a reliable technique that can substantially reduce invasive prenatal tests. (C) 2004 by The American College of Obstetricians and Gynecologists
AB - OBJECTIVE: To describe our clinical experience with detection and analysis of cell-free fetal DNA derived from maternal plasma for prenatal sexing and fetal rhesus-D typing. METHODS: Real-time quantitative polymerase chain reactions (PCRs) of rhesus-D sequences and the SRY gene were validated and offered to patients with an enhanced risk for sex-linked fetal pathology and patients with rhesus-D antibodies. RESULTS: In die validation group, 72 samples were analyzed. Sensitivity of the rhesus-D real-time quantitative PCR in maternal plasma was 100% (95% confidence interval [CI]91.8%, 100%) and specificity was 96.6% (95% CI 82.2%,99.9%). Sensitivity of the SRY real-time quantitative PCR was 97.2% (95% CI 85.5%, 99.9%), and specificity was 100% (95% CI 88.1%, 100%). The technique was used successfully in a clinical setting in 24 women. Overall, invasive tests were avoided in 41.7% of these patients. CONCLUSION: Detection of cell-free fetal DNA from maternal plasma is a reliable technique that can substantially reduce invasive prenatal tests. (C) 2004 by The American College of Obstetricians and Gynecologists
U2 - https://doi.org/10.1097/01.AOG.0000103996.44503.F1
DO - https://doi.org/10.1097/01.AOG.0000103996.44503.F1
M3 - Article
C2 - 14704260
SN - 0029-7844
VL - 103
SP - 157
EP - 164
JO - Obstetrics and Gynecology
JF - Obstetrics and Gynecology
IS - 1
ER -