TY - JOUR
T1 - Co-isolation of extracellular vesicles and high-density lipoproteins using density gradient ultracentrifugation
AU - Yuana, Yuana
AU - Levels, Johannes
AU - Grootemaat, Anita
AU - Sturk, Auguste
AU - Nieuwland, Rienk
PY - 2014
Y1 - 2014
N2 - Extracellular vesicles (EVs) facilitate intercellular communication by carrying bioactive molecules such as proteins, messenger RNA, and micro (mi)RNAs. Recently, high-density lipoproteins (HDL) isolated from human plasma were also reported to transport miRNA to other cells. HDL, when isolated from human plasma, ranges in density between 1.063 and 1.21 g/mL, which grossly overlap with the reported density of EVs. Consequently, HDL and EV will be co-isolated when using density gradient ultracentrifugation. Thus, more stringent isolation/separation procedures of EV and HDL are essential to know their relative contribution to the pool of circulating bioactive molecules
AB - Extracellular vesicles (EVs) facilitate intercellular communication by carrying bioactive molecules such as proteins, messenger RNA, and micro (mi)RNAs. Recently, high-density lipoproteins (HDL) isolated from human plasma were also reported to transport miRNA to other cells. HDL, when isolated from human plasma, ranges in density between 1.063 and 1.21 g/mL, which grossly overlap with the reported density of EVs. Consequently, HDL and EV will be co-isolated when using density gradient ultracentrifugation. Thus, more stringent isolation/separation procedures of EV and HDL are essential to know their relative contribution to the pool of circulating bioactive molecules
U2 - https://doi.org/10.3402/jev.v3.23262
DO - https://doi.org/10.3402/jev.v3.23262
M3 - Article
C2 - 25018865
SN - 2001-3078
VL - 3
SP - 23262
JO - Journal of extracellular vesicles
JF - Journal of extracellular vesicles
ER -