Core alpha1-->3-fucose is a common modification of N-glycans in parasitic helminths and constitutes an important epitope for IgE from Haemonchus contortus infected sheep

I. van Die, V. Gomord, F. N. Kooyman, T. K. van den Berg, R. D. Cummings, L. Vervelde

Research output: Contribution to journalArticleAcademicpeer-review

115 Citations (Scopus)

Abstract

Synthesis of parasite specific IgE plays a critical role in the defence against helminth infections. We report here that IgE from serum from Schistosoma mansoni infected mice and Haemonchus contortus infected sheep recognizes complex-type N-glycans from Arabidopsis thaliana, which contain R-GlcNAcbeta1-->4(Fucalpha1-->3)GlcNAcbeta1-Asn (core alpha1-->3-Fuc) and Xylbeta1-->2Manbeta1-->4GlcNAcbeta1-R (core beta1-->2-Xyl) modifications, and honeybee phospholipase A2, which carries N-glycans that contain the core alpha1-->3-Fuc epitope. Evidence is presented that core alpha1-->3-fucosylated N-glycans bind a substantial part of the parasite specific IgE in serum of H. contortus infected sheep. These results suggest that the core alpha1-->3-Fuc antigen may contribute to induction of a Th2 response leading to the production of IgE. In addition we show here that N-glycans carrying core alpha1-->3-Fuc and beta1-->2-Xyl antigens are synthesized by many parasitic helminths and also by the free living nematode Caenorhabditis elegans. Since N-glycans containing the core alpha1-->3-Fuc have also been implicated in honeybee and plant induced allergies, this conserved glycan might represent an important common IgE epitope
Original languageEnglish
Pages (from-to)189-193
Number of pages5
JournalFEBS letters
Volume463
Issue number1-2
DOIs
Publication statusPublished - 10 Dec 1999

Keywords

  • Animals
  • Arabidopsis/chemistry
  • Binding, Competitive
  • Blotting, Western
  • Caenorhabditis elegans/chemistry
  • Dose-Response Relationship, Drug
  • Enzyme-Linked Immunosorbent Assay
  • Epitopes/immunology
  • Fucose/chemistry
  • Haemonchiasis/immunology
  • Haemonchus/chemistry
  • Immunoglobulin E/blood
  • Immunoglobulin G/blood
  • Mice
  • Polysaccharides/chemistry
  • Schistosoma mansoni/chemistry
  • Sheep/immunology
  • Substrate Specificity

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