TY - JOUR
T1 - Cutaneous leishmaniosis in a horse from northern Portugal
AU - Gama, Adelina
AU - Elias, Joana
AU - Ribeiro, Ana J.
AU - Alegria, Nuno
AU - Schallig, Henk D. F. H.
AU - Silva, Filipe
AU - Santarém, Nuno
AU - Cardoso, Luís
AU - Cotovio, Mário
PY - 2014
Y1 - 2014
N2 - The first case of cutaneous leishmaniosis in a horse from the north of Portugal, with a 1.5 cm in diameter ulcerated nodular lesion on the left face, is reported. The skin nodule was surgically excised and assessed by histopathology, including an immunohistochemistiy method applied for the first time to equine tissues which clearly demonstrated leishmanial amastigote forms. Two serological determinations with the direct agglutination test performed 13 months apart showed seroconversion specific for Leishmania from a <25 to a 200 antibody titre. Polymerase chain reaction followed by kinetoplast DNA sequencing provided a 116-bp sequence with 98% identity to Leishmania infantum closest sequence deposited in GenBank. No recurrence was observed after complete surgical excision. Leishmaniosis should be included in the differential diagnosis of cutaneous nodular or papular lesions in the equine species in Portugal. (C) 2013 Elsevier B.V. All rights reserved
AB - The first case of cutaneous leishmaniosis in a horse from the north of Portugal, with a 1.5 cm in diameter ulcerated nodular lesion on the left face, is reported. The skin nodule was surgically excised and assessed by histopathology, including an immunohistochemistiy method applied for the first time to equine tissues which clearly demonstrated leishmanial amastigote forms. Two serological determinations with the direct agglutination test performed 13 months apart showed seroconversion specific for Leishmania from a <25 to a 200 antibody titre. Polymerase chain reaction followed by kinetoplast DNA sequencing provided a 116-bp sequence with 98% identity to Leishmania infantum closest sequence deposited in GenBank. No recurrence was observed after complete surgical excision. Leishmaniosis should be included in the differential diagnosis of cutaneous nodular or papular lesions in the equine species in Portugal. (C) 2013 Elsevier B.V. All rights reserved
U2 - https://doi.org/10.1016/j.vetpar.2013.12.005
DO - https://doi.org/10.1016/j.vetpar.2013.12.005
M3 - Article
C2 - 24388338
SN - 0304-4017
VL - 200
SP - 189
EP - 192
JO - Veterinary Parasitology
JF - Veterinary Parasitology
IS - 1-2
ER -