Cutting edge: carbohydrate profiling identifies new pathogens that interact with dendritic cell-specific ICAM-3-grabbing nonintegrin on dendritic cells: carbohydrate profiling identifies new pathogens that interact with dendritic cell-specific ICAM-3-grabbing nonintegrin on dendritic cells

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Abstract

Dendritic cells (DC) are instrumental in handling pathogens for processing and presentation to T cells, thus eliciting an appropriate immune response. C-type lectins expressed by DC function as pathogen-recognition receptors; yet their specificity for carbohydrate structures on pathogens is not fully understood. In this study, we analyzed the carbohydrate specificity of DC-specific ICAM-3-grabbing nonintegrin (SIGN)/CD209, the recently documented HIV-1 receptor on DC. Our studies show that DC-SIGN binds with high affinity to both synthetic mannose- and fucose-containing glycoconjugates. These carbohydrate structures are abundantly expressed by pathogens as demonstrated by the affinity of DC-SIGN for natural surface glycans of the human pathogens Mycobacterium tuberculosis, Helicobacter pylori, Leishmania mexicana, and Schistosoma mansoni. This analysis expands our knowledge on the carbohydrate and pathogen-specificity of DC-SIGN and identifies this lectin to be central in pathogen-DC interactions
Original languageEnglish
Pages (from-to)1635-1639
Number of pages5
JournalJournal of immunology (Baltimore, Md.
Volume170
Issue number4
DOIs
Publication statusPublished - 15 Feb 2003

Keywords

  • Animals
  • CHO Cells
  • Carbohydrate Metabolism
  • Carbohydrate Sequence
  • Carbohydrates/immunology
  • Cell Adhesion Molecules/immunology
  • Cell Adhesion/immunology
  • Cells, Cultured
  • Cricetinae
  • Dendritic Cells/immunology
  • Fucose/immunology
  • Helicobacter pylori/immunology
  • Humans
  • K562 Cells
  • Lectins, C-Type/immunology
  • Leishmania mexicana/immunology
  • Mannose/immunology
  • Molecular Sequence Data
  • Mycobacterium tuberculosis/immunology
  • Protein Binding/immunology
  • Receptors, Cell Surface/immunology
  • Recombinant Fusion Proteins/immunology
  • Schistosoma mansoni/immunology
  • Transfection

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