TY - JOUR
T1 - Cyclosporine A administered during reperfusion fails to restore cardioprotection in prediabetic Zucker obese rats in vivo
AU - Huhn, R.
AU - Heinen, A.
AU - Hollmann, M. W.
AU - Schlack, W.
AU - Preckel, B.
AU - Weber, N. C.
PY - 2010
Y1 - 2010
N2 - Background and aims: Hyperglycaemia blocks sevoflurane-induced postconditioning, and cardioprotection in hyperglycaemic myocardium can be restored by inhibition of the mitochondrial permeability transition pore (mPTP). We investigated whether sevoflurane-induced postconditioning is also blocked in the prediabetic heart and if so, whether cardioprotection could be restored by inhibiting mPTP. Methods and results: Zucker lean (ZL) and Zucker obese (ZO) rats were assigned to one of seven groups. Animals underwent 25 min of ischaemia and 120 min of reperfusion. Control (ZL-/ZO Con) animals were not further treated. postconditioning groups (ZL-/ZO Sevo-post) received sevoflurane for 5 min starting 1 min prior to the onset of reperfusion. The mPTP inhibitor cyclosporine A (CsA) was administered intravenously in a concentration of 5 (ZO CsA and ZO CsA + Sevo-post) or 10 mg/kg (ZO CsA10 + Sevo-post) 5 min before the onset of reperfusion. At the end of reperfusion, infarct sizes were measured by TTC staining. Blood samples were collected to measure plasma levels of insulin, cholesterol and triglycerides. Sevoflurane postconditioning reduced infarct size in ZL rats to 35 +/- 12% (p <0.05 vs. ZL Con: 60 +/- 6%). In ZO rats sevoflurane postconditioning was abolished (ZO Sevo-post: 59 +/- 12%, n.s. vs. ZO Con: 58 +/- 6%). 5 mg and 10 mg CsA could not restore cardioprotection (ZO CsA + Sevo-post: 59 +/- 7%, ZO CsA10 + Sevo-post: 57 +/- 14%; n.s. vs. ZO Con). In ZO rats insulin, cholesterol and triglyceride levels were significant higher than in ZL rats (all p <0.05). Conclusion: Inhibition of mPTP with CsA failed to restore cardioprotection in the prediabetic but normoglycaemic heart of Zucker obese rats in vivo. (C) 2009 Elsevier B.V. All rights reserved
AB - Background and aims: Hyperglycaemia blocks sevoflurane-induced postconditioning, and cardioprotection in hyperglycaemic myocardium can be restored by inhibition of the mitochondrial permeability transition pore (mPTP). We investigated whether sevoflurane-induced postconditioning is also blocked in the prediabetic heart and if so, whether cardioprotection could be restored by inhibiting mPTP. Methods and results: Zucker lean (ZL) and Zucker obese (ZO) rats were assigned to one of seven groups. Animals underwent 25 min of ischaemia and 120 min of reperfusion. Control (ZL-/ZO Con) animals were not further treated. postconditioning groups (ZL-/ZO Sevo-post) received sevoflurane for 5 min starting 1 min prior to the onset of reperfusion. The mPTP inhibitor cyclosporine A (CsA) was administered intravenously in a concentration of 5 (ZO CsA and ZO CsA + Sevo-post) or 10 mg/kg (ZO CsA10 + Sevo-post) 5 min before the onset of reperfusion. At the end of reperfusion, infarct sizes were measured by TTC staining. Blood samples were collected to measure plasma levels of insulin, cholesterol and triglycerides. Sevoflurane postconditioning reduced infarct size in ZL rats to 35 +/- 12% (p <0.05 vs. ZL Con: 60 +/- 6%). In ZO rats sevoflurane postconditioning was abolished (ZO Sevo-post: 59 +/- 12%, n.s. vs. ZO Con: 58 +/- 6%). 5 mg and 10 mg CsA could not restore cardioprotection (ZO CsA + Sevo-post: 59 +/- 7%, ZO CsA10 + Sevo-post: 57 +/- 14%; n.s. vs. ZO Con). In ZO rats insulin, cholesterol and triglyceride levels were significant higher than in ZL rats (all p <0.05). Conclusion: Inhibition of mPTP with CsA failed to restore cardioprotection in the prediabetic but normoglycaemic heart of Zucker obese rats in vivo. (C) 2009 Elsevier B.V. All rights reserved
U2 - https://doi.org/10.1016/j.numecd.2009.06.010
DO - https://doi.org/10.1016/j.numecd.2009.06.010
M3 - Article
C2 - 19819119
SN - 0939-4753
VL - 20
SP - 706
EP - 712
JO - Nutrition, metabolism, and cardiovascular diseases
JF - Nutrition, metabolism, and cardiovascular diseases
IS - 10
ER -