TY - JOUR
T1 - Cytokine signatures in chronic fatigue syndrome patients: a Case Control Study and the effect of anakinra treatment
AU - Roerink, Megan E.
AU - Knoop, Hans
AU - Bronkhorst, Ewald M.
AU - Mouthaan, Henk A.
AU - Hawinkels, Luuk J. A. C.
AU - Joosten, Leo A. B.
AU - van der Meer, Jos W. M.
PY - 2017
Y1 - 2017
N2 - Background: Cytokine disturbances have been suggested to be associated with the Chronic Fatigue Syndrome/Myalgic encephalomyelitis (CFS/ME) for decades. Methods: Fifty female CFS patients were included in a study on the effect of the interleukin-1-receptor antagonist anakinra or placebo during 4 weeks. EDTA plasma was collected from patients before and directly after treatment. At baseline, plasma samples were collected at the same time from 48 healthy, age-matched female neighborhood controls. A panel of 92 inflammatory markers was determined in parallel in 1 mu L samples using a 'proximity extension assay' (PEA) based immunoassay. Since Transforming growth factor beta (TGF-beta) and interleukin-1 receptor antagonist (IL-1Ra) were not included in this platform, these cytokines were measured with ELISA. Results: In CFS/ME patients, the 'normalized protein expression' value of IL-12p40 and CSF-1 was significantly higher (p value 0.0042 and 0.049, respectively). Furthermore, using LASSO regression, a combination of 47 markers yielded a prediction model with a corrected AUC of 0.73. After correction for multiple testing, anakinra had no effect on circulating cytokines. TGF-beta did not differ between patients and controls. Conclusions: In conclusion, this study demonstrated increased IL-12p40 and CSF-1 concentrations in CFS/ME patients in addition to a set of predictive biomarkers. There was no effect of anakinra on circulating cytokines other than IL-1Ra
AB - Background: Cytokine disturbances have been suggested to be associated with the Chronic Fatigue Syndrome/Myalgic encephalomyelitis (CFS/ME) for decades. Methods: Fifty female CFS patients were included in a study on the effect of the interleukin-1-receptor antagonist anakinra or placebo during 4 weeks. EDTA plasma was collected from patients before and directly after treatment. At baseline, plasma samples were collected at the same time from 48 healthy, age-matched female neighborhood controls. A panel of 92 inflammatory markers was determined in parallel in 1 mu L samples using a 'proximity extension assay' (PEA) based immunoassay. Since Transforming growth factor beta (TGF-beta) and interleukin-1 receptor antagonist (IL-1Ra) were not included in this platform, these cytokines were measured with ELISA. Results: In CFS/ME patients, the 'normalized protein expression' value of IL-12p40 and CSF-1 was significantly higher (p value 0.0042 and 0.049, respectively). Furthermore, using LASSO regression, a combination of 47 markers yielded a prediction model with a corrected AUC of 0.73. After correction for multiple testing, anakinra had no effect on circulating cytokines. TGF-beta did not differ between patients and controls. Conclusions: In conclusion, this study demonstrated increased IL-12p40 and CSF-1 concentrations in CFS/ME patients in addition to a set of predictive biomarkers. There was no effect of anakinra on circulating cytokines other than IL-1Ra
U2 - https://doi.org/10.1186/s12967-017-1371-9
DO - https://doi.org/10.1186/s12967-017-1371-9
M3 - Article
C2 - 29284500
SN - 1479-5876
VL - 15
SP - 267
JO - Journal of translational medicine
JF - Journal of translational medicine
IS - 1
ER -