Cytokines in aqueous humour and serum before and after corneal transplantation and during rejection

B. E. van Gelderen; A. van der Lelij; R. Peek; L. Broersma; W. F. Treffers; J. M. Ruijter; R. van der Gaag

doi:https://doi.org/10.1159/000055607

Cytokines in aqueous humour and serum before and after corneal transplantation and during rejection

B. E. van Gelderen, A. van der Lelij, R. Peek, L. Broersma, W. F. Treffers, J. M. Ruijter, R. van der Gaag

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Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

Cytokine profiles in aqueous humour were studied in relation to corneal disease and subsequent corneal graft survival or rejection. Cytokine levels in samples obtained from eyes with clear grafts (n = 59) were all within the normal range. At the time of penetrating keratoplasty (n = 146), intraocular levels of IL-6 were increased in 38% (50/131), most markedly in eyes with previous allograft failure or herpetic stromal keratitis. The level of IL-10 was increased in 1 eye (n = 144) and of IL-4 and IFN-gamma in none. During rejection (n = 10), the levels of IL-6 in aqueous humour were increased in 75% (3/4), of IL-10 in 50% (3/6), of IL-4 in none (0/4) and of IFN-gamma in 40% (2/5). In conclusion, the levels of total protein and IL-6 were increased prior to penetrating keratoplasty in eyes with previous inflammation. These results could however not predict the final outcome of the graft. Increased intraocular levels of IL-6, IL-10 and IFN-gamma were observed during rejection

Original language	English
Pages (from-to)	157-164
Journal	Ophthalmic research
Volume	32
Issue number	4
DOIs	https://doi.org/10.1159/000055607
Publication status	Published - 2000

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https://doi.org/10.1159/000055607

Cite this

@article{16e7b3ea63a44797b91cafde01ab910c,

title = "Cytokines in aqueous humour and serum before and after corneal transplantation and during rejection",

abstract = "Cytokine profiles in aqueous humour were studied in relation to corneal disease and subsequent corneal graft survival or rejection. Cytokine levels in samples obtained from eyes with clear grafts (n = 59) were all within the normal range. At the time of penetrating keratoplasty (n = 146), intraocular levels of IL-6 were increased in 38% (50/131), most markedly in eyes with previous allograft failure or herpetic stromal keratitis. The level of IL-10 was increased in 1 eye (n = 144) and of IL-4 and IFN-gamma in none. During rejection (n = 10), the levels of IL-6 in aqueous humour were increased in 75% (3/4), of IL-10 in 50% (3/6), of IL-4 in none (0/4) and of IFN-gamma in 40% (2/5). In conclusion, the levels of total protein and IL-6 were increased prior to penetrating keratoplasty in eyes with previous inflammation. These results could however not predict the final outcome of the graft. Increased intraocular levels of IL-6, IL-10 and IFN-gamma were observed during rejection",

author = "{van Gelderen}, {B. E.} and {van der Lelij}, A. and R. Peek and L. Broersma and Treffers, {W. F.} and Ruijter, {J. M.} and {van der Gaag}, R.",

year = "2000",

doi = "https://doi.org/10.1159/000055607",

language = "English",

volume = "32",

pages = "157--164",

journal = "Ophthalmic research",

issn = "0030-3747",

publisher = "Karger",

number = "4",

}

TY - JOUR

T1 - Cytokines in aqueous humour and serum before and after corneal transplantation and during rejection

AU - van Gelderen, B. E.

AU - van der Lelij, A.

AU - Peek, R.

AU - Broersma, L.

AU - Treffers, W. F.

AU - Ruijter, J. M.

AU - van der Gaag, R.

PY - 2000

Y1 - 2000

N2 - Cytokine profiles in aqueous humour were studied in relation to corneal disease and subsequent corneal graft survival or rejection. Cytokine levels in samples obtained from eyes with clear grafts (n = 59) were all within the normal range. At the time of penetrating keratoplasty (n = 146), intraocular levels of IL-6 were increased in 38% (50/131), most markedly in eyes with previous allograft failure or herpetic stromal keratitis. The level of IL-10 was increased in 1 eye (n = 144) and of IL-4 and IFN-gamma in none. During rejection (n = 10), the levels of IL-6 in aqueous humour were increased in 75% (3/4), of IL-10 in 50% (3/6), of IL-4 in none (0/4) and of IFN-gamma in 40% (2/5). In conclusion, the levels of total protein and IL-6 were increased prior to penetrating keratoplasty in eyes with previous inflammation. These results could however not predict the final outcome of the graft. Increased intraocular levels of IL-6, IL-10 and IFN-gamma were observed during rejection

AB - Cytokine profiles in aqueous humour were studied in relation to corneal disease and subsequent corneal graft survival or rejection. Cytokine levels in samples obtained from eyes with clear grafts (n = 59) were all within the normal range. At the time of penetrating keratoplasty (n = 146), intraocular levels of IL-6 were increased in 38% (50/131), most markedly in eyes with previous allograft failure or herpetic stromal keratitis. The level of IL-10 was increased in 1 eye (n = 144) and of IL-4 and IFN-gamma in none. During rejection (n = 10), the levels of IL-6 in aqueous humour were increased in 75% (3/4), of IL-10 in 50% (3/6), of IL-4 in none (0/4) and of IFN-gamma in 40% (2/5). In conclusion, the levels of total protein and IL-6 were increased prior to penetrating keratoplasty in eyes with previous inflammation. These results could however not predict the final outcome of the graft. Increased intraocular levels of IL-6, IL-10 and IFN-gamma were observed during rejection

U2 - https://doi.org/10.1159/000055607

DO - https://doi.org/10.1159/000055607

M3 - Article

C2 - 10828736

SN - 0030-3747

VL - 32

SP - 157

EP - 164

JO - Ophthalmic research

JF - Ophthalmic research

IS - 4

ER -