@article{d35fbba8d45a42f4b7a1d289a5ca7a1c,
title = "Benchmarking blood collection tubes and processing intervals for extracellular vesicle performance metrics",
abstract = "The analysis of extracellular vesicles (EV) in blood samples is under intense investigation and holds the potential to deliver clinically meaningful biomarkers for health and disease. Technical variation must be minimized to confidently assess EV-associated biomarkers, but the impact of pre-analytics on EV characteristics in blood samples remains minimally explored. We present the results from the first large-scale EV Blood Benchmarking (EVBB) study in which we systematically compared 11 blood collection tubes (BCT; six preservation and five non-preservation) and three blood processing intervals (BPI; 1, 8 and 72 h) on defined performance metrics (n = 9). The EVBB study identifies a significant impact of multiple BCT and BPI on a diverse set of metrics reflecting blood sample quality, ex-vivo generation of blood-cell derived EV, EV recovery and EV-associated molecular signatures. The results assist the informed selection of the optimal BCT and BPI for EV analysis. The proposed metrics serve as a framework to guide future research on pre-analytics and further support methodological standardization of EV studies.",
keywords = "RNA sequencing, anticoagulants, biomarkers, cancer, exosomes, extracellular vesicles, plasma, preservatives, proteomics, serum",
author = "Bert Dhondt and Cl{\'a}udio Pinheiro and Edward Geeurickx and Joeri Tulkens and Glenn Vergauwen and {van der Pol}, Edwin and Rienk Nieuwland and Anneleen Decock and Ilkka Miinalainen and Pekka Rappu and Gary Schroth and Scott Kuersten and Jo Vandesompele and Pieter Mestdagh and Nicolaas Lumen and {de Wever}, Olivier and An Hendrix",
note = "Funding Information: We thank Sofie De Geyter (Laboratory of Experimental Cancer Research, Department of Human Structure and Repair, Ghent University, Ghent, Belgium), and Najat Hajji and Chi Hau (Laboratory of Experimental Clinical Chemistry, Amsterdam UCM, location AMC, University of Amsterdam, Amsterdam, The Netherlands) for experimental support. We also thank the proteomics and electron microscopy core facilities, supported by biocenter Finland and, respectively, the University of Turku (Finland) and University of Oulu (Finland). This work was supported by the Fund for Scientific Spearheads of Ghent University Hospital, Concerted Research Actions from Ghent University, “Stichting tegen Kanker,” the Fund for Scientific Research‐Flanders, and “Kom op tegen Kanker (Stand up to Cancer), the Flemisch cancer society”. Funding Information: We thank Sofie De Geyter (Laboratory of Experimental Cancer Research, Department of Human Structure and Repair, Ghent University, Ghent, Belgium), and Najat Hajji and Chi Hau (Laboratory of Experimental Clinical Chemistry, Amsterdam UCM, location AMC, University of Amsterdam, Amsterdam, The Netherlands) for experimental support. We also thank the proteomics and electron microscopy core facilities, supported by biocenter Finland and, respectively, the University of Turku (Finland) and University of Oulu (Finland). This work was supported by the Fund for Scientific Spearheads of Ghent University Hospital, Concerted Research Actions from Ghent University, “Stichting tegen Kanker,” the Fund for Scientific Research-Flanders, and “Kom op tegen Kanker (Stand up to Cancer), the Flemisch cancer society”. Publisher Copyright: {\textcopyright} 2023 The Authors. Journal of Extracellular Vesicles published by Wiley Periodicals, LLC on behalf of the International Society for Extracellular Vesicles.",
year = "2023",
month = may,
day = "1",
doi = "https://doi.org/10.1002/jev2.12315",
language = "English",
volume = "12",
journal = "Journal of extracellular vesicles",
issn = "2001-3078",
publisher = "Taylor & Francis",
number = "5",
}