TY - JOUR
T1 - DAMPS and complement activation in platelet concentrates that induce adverse reactions in patients
AU - de Wit, Yasmin E. S.
AU - Hamzeh-Cognasse, Hind
AU - Cognasse, Fabrice
AU - ten Brinke, Anja
AU - Zeerleder, Sacha S.
N1 - Funding Information: Stichting Sanquin Bloedvoorziening, Grant/Award Number: PPOC Grant 17-44 Publisher Copyright: © 2022 AABB.
PY - 2022/9
Y1 - 2022/9
N2 - Background: Patients with severe thrombocytopenia due to bone marrow failure and after chemotherapy are still treated with platelet transfusions. Platelet concentrates (PC) are associated with a high incidence of adverse reactions (AR). Platelet-derived damage-associated molecular patterns (DAMPS) and complement were proposed to play a role in the pathology of AR. Study Design and Methods: Single donor apheresis platelet concentrates (SDA PCs) were produced in a regional setting of the French Blood Establishment. After transfusion samples were collected from PC and possible AR in patients were recorded. Platelet activation markers, High mobility group box 1 (HMGB1) and complement activation products (CAP) were measured. The correlation between platelet activation, and HMGB1 and complement activation was analyzed. Results: A total of 56 PC were included in the study. 30 PC induced no AR, and 26 induced AR (Febrile non-hemolytic transfusion reaction n = 16; Atypical Allergic Transfusion Reactions n = 11; hemodynamic instability n = 5) in the patients. The levels of P-selectin, sCD40L, HMGB1, C3b/c, and C4b/c were all significantly increased in PC that induced AR following transfusion in patients. Additionally, HMGB1, C3b/c, and C4b/c were positively correlated with P-selectin and sCD40L. Conclusion: In this study, we observed an association between HMGB1 and CAP and the incidence of AR. Furthermore, we demonstrated that both HMGB1 and complement activation were correlated to platelet activation.
AB - Background: Patients with severe thrombocytopenia due to bone marrow failure and after chemotherapy are still treated with platelet transfusions. Platelet concentrates (PC) are associated with a high incidence of adverse reactions (AR). Platelet-derived damage-associated molecular patterns (DAMPS) and complement were proposed to play a role in the pathology of AR. Study Design and Methods: Single donor apheresis platelet concentrates (SDA PCs) were produced in a regional setting of the French Blood Establishment. After transfusion samples were collected from PC and possible AR in patients were recorded. Platelet activation markers, High mobility group box 1 (HMGB1) and complement activation products (CAP) were measured. The correlation between platelet activation, and HMGB1 and complement activation was analyzed. Results: A total of 56 PC were included in the study. 30 PC induced no AR, and 26 induced AR (Febrile non-hemolytic transfusion reaction n = 16; Atypical Allergic Transfusion Reactions n = 11; hemodynamic instability n = 5) in the patients. The levels of P-selectin, sCD40L, HMGB1, C3b/c, and C4b/c were all significantly increased in PC that induced AR following transfusion in patients. Additionally, HMGB1, C3b/c, and C4b/c were positively correlated with P-selectin and sCD40L. Conclusion: In this study, we observed an association between HMGB1 and CAP and the incidence of AR. Furthermore, we demonstrated that both HMGB1 and complement activation were correlated to platelet activation.
KW - DAMPS
KW - HMGB1
KW - complement activation
KW - febrile non-hemolytic reaction
KW - platelet concentrates
KW - transfusion
KW - transfusion adverse reactions
UR - http://www.scopus.com/inward/record.url?scp=85135629271&partnerID=8YFLogxK
U2 - https://doi.org/10.1111/trf.17061
DO - https://doi.org/10.1111/trf.17061
M3 - Article
C2 - 35950480
SN - 0041-1132
VL - 62
SP - 1721
EP - 1726
JO - Transfusion
JF - Transfusion
IS - 9
ER -