Design, measurement and processing of region-specific DNA methylation assays: The mass spectrometry-based method EpiTYPER

H. Eka D. Suchiman; Roderick C. Slieker; Dennis Kremer; P. Eline Slagboom; Bastiaan T. Heijmans; Elmar W. Tobi

doi:https://doi.org/10.3389/fgene.2015.00287

Design, measurement and processing of region-specific DNA methylation assays: The mass spectrometry-based method EpiTYPER

H. Eka D. Suchiman, Roderick C. Slieker, Dennis Kremer, P. Eline Slagboom, Bastiaan T. Heijmans, Elmar W. Tobi

Research output: Contribution to journal › Article › Academic › peer-review

69 Citations (Scopus)

Abstract

EpiTYPER® is a mass spectrometry-based bisulfite sequencing method that enables region-specific DNA methylation analysis in a quantitative and high-throughput fashion. The technology targets genomic regions of 100-600 base pairs and results in the quantitative measurement of DNA methylation levels largely at single-nucleotide resolution. It is particularly suitable for larger scale efforts to study candidate regions or to validate regions from genome-wide DNA methylation studies. Here, we describe in detail how to design and perform EpiTYPER measurements and preprocess the data, providing details for high quality measurements not provided in the standard EpiTYPER protocol.

Original language	English
Article number	287
Journal	Frontiers in genetics
Volume	6
Issue number	SEP
DOIs	https://doi.org/10.3389/fgene.2015.00287
Publication status	Published - 1 Jan 2015

Keywords

Bisulfite
DNA methylation
EpiTYPER®
Mass spectrometry
Quantitative
Region-specific

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https://doi.org/10.3389/fgene.2015.00287

Cite this

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AU - Tobi, Elmar W.

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Design, measurement and processing of region-specific DNA methylation assays: The mass spectrometry-based method EpiTYPER

Abstract

Keywords

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