Detection of human IgM and IgG antibodies by means of galactofuranose-coated and rhamnose-coated gold nanoparticles

Xinyue Wang; Huaiyong Chen; F Chiodo; Boris Tefsen

Detection of human IgM and IgG antibodies by means of galactofuranose-coated and rhamnose-coated gold nanoparticles

Xinyue Wang, Huaiyong Chen, F Chiodo, Boris Tefsen

Molecular cell biology and Immunology (VUmc)

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Many infectious diseases lack cheap, fast, and reliable serodiagnostic tools. Recently, glycans coupled to gold nanoparticles (GNPs) have shown potential to fill this void by utilizing them as a coating in ELISA experiments. In this study, we used GNPs functionalized either with galactofuranose (Galf), rhamnose (Rhap) or a mixture of these monosaccharides to detect the presence of IgM and IgG antibodies against these non-human carbohydrates in sera obtained from tuberculosis patients and control groups. Our findings confirm the wide abundance of anti-Rhap antibodies in humans and show for the first time the presence of anti-Galf antibodies in human sera. This study supports further investigation of using GNP-ELISA with microbe-specific carbohydrates for serodiagnosis of infectious diseases.

Original language	English
Journal	Matters
Publication status	Published - 30 Aug 2019

Cite this

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title = "Detection of human IgM and IgG antibodies by means of galactofuranose-coated and rhamnose-coated gold nanoparticles",

abstract = "Many infectious diseases lack cheap, fast, and reliable serodiagnostic tools. Recently, glycans coupled to gold nanoparticles (GNPs) have shown potential to fill this void by utilizing them as a coating in ELISA experiments. In this study, we used GNPs functionalized either with galactofuranose (Galf), rhamnose (Rhap) or a mixture of these monosaccharides to detect the presence of IgM and IgG antibodies against these non-human carbohydrates in sera obtained from tuberculosis patients and control groups. Our findings confirm the wide abundance of anti-Rhap antibodies in humans and show for the first time the presence of anti-Galf antibodies in human sera. This study supports further investigation of using GNP-ELISA with microbe-specific carbohydrates for serodiagnosis of infectious diseases.",

author = "Xinyue Wang and Huaiyong Chen and F Chiodo and Boris Tefsen",

year = "2019",

month = aug,

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language = "English",

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T1 - Detection of human IgM and IgG antibodies by means of galactofuranose-coated and rhamnose-coated gold nanoparticles

AU - Wang, Xinyue

AU - Chen, Huaiyong

AU - Chiodo, F

AU - Tefsen, Boris

PY - 2019/8/30

Y1 - 2019/8/30

N2 - Many infectious diseases lack cheap, fast, and reliable serodiagnostic tools. Recently, glycans coupled to gold nanoparticles (GNPs) have shown potential to fill this void by utilizing them as a coating in ELISA experiments. In this study, we used GNPs functionalized either with galactofuranose (Galf), rhamnose (Rhap) or a mixture of these monosaccharides to detect the presence of IgM and IgG antibodies against these non-human carbohydrates in sera obtained from tuberculosis patients and control groups. Our findings confirm the wide abundance of anti-Rhap antibodies in humans and show for the first time the presence of anti-Galf antibodies in human sera. This study supports further investigation of using GNP-ELISA with microbe-specific carbohydrates for serodiagnosis of infectious diseases.

AB - Many infectious diseases lack cheap, fast, and reliable serodiagnostic tools. Recently, glycans coupled to gold nanoparticles (GNPs) have shown potential to fill this void by utilizing them as a coating in ELISA experiments. In this study, we used GNPs functionalized either with galactofuranose (Galf), rhamnose (Rhap) or a mixture of these monosaccharides to detect the presence of IgM and IgG antibodies against these non-human carbohydrates in sera obtained from tuberculosis patients and control groups. Our findings confirm the wide abundance of anti-Rhap antibodies in humans and show for the first time the presence of anti-Galf antibodies in human sera. This study supports further investigation of using GNP-ELISA with microbe-specific carbohydrates for serodiagnosis of infectious diseases.

M3 - Article

SN - 2297-8240

JO - Matters

JF - Matters

ER -