Determination of natural moisturizing factors in the skin: Raman microspectroscopy versus HPLC

S. A. Koppes, P. Kemperman, I. Van Tilburg, F. Calkoen-Kwa, K. A. Engebretsen, G. J. Puppels, P. J. Caspers, S. Kezic

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23 Citations (Scopus)


Background: Natural moisturizing factor (NMF) is used as genotypic and phenotypic biomarker in diagnostics. This study is a side-to-side comparison of two different methods to determine NMF in atopic dermatitis patients: Raman microspectroscopy and stratum corneum tape stripping followed by HPLC. Results: Measured NMF values were significantly correlated (R2 =.61; p <.0001), both methods demonstrated a concentration-depth dependence of NMF and reduced NMF levels in the carriers of filaggrin null mutations. Good agreement between measurements of left and right arms indicated robustness and good reproducibility of both methods. Conclusions: Both methods showed comparable performance, choice of method will rather be influenced by practical consideration.

Original languageEnglish
Pages (from-to)502-507
Number of pages6
Issue number6
Early online date2016
Publication statusPublished - 18 Aug 2017


  • Dermatology
  • HPCL and Raman microspectroscopy
  • NMF

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