Developmental apoptosis in the spinal cord white matter in neonatal rats

A J A De Louw, W D J Van De Berg, J De Vente, C E Blanco, A W D Gavilanes, H P J Steinbusch, H W M Steinbusch, J Troost, J S H Vles

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12 Citations (Scopus)


We investigated developmental apoptosis in the white matter of the cervical spinal cord at postnatal days 2, 5, and 8. Apoptotic cells were labeled using TUNEL and caspase-3 immunostaining. Apoptotic cells were diffusely distributed throughout the white matter of the spinal cord. The total amount of apoptotic cells in the cervical spinal cord white matter was related to postnatal age, with the lowest at P2 (mean 7.9, SD 5.6) and the highest at P8 (mean 109, SD 21.4). Using double immunostaining for ED-1 and O4, apoptotic cells were identified as microglia and oligodendrocytes.

Original languageEnglish
Pages (from-to)89-91
Number of pages3
Issue number1
Publication statusPublished - Jan 2002


  • Aging/physiology
  • Animals
  • Apoptosis/physiology
  • Caspase 3
  • Caspases/metabolism
  • Ectodysplasins
  • In Situ Nick-End Labeling
  • Membrane Proteins/metabolism
  • Microglia/cytology
  • Nerve Fibers, Myelinated/metabolism
  • Oligodendroglia/cytology
  • Rats
  • Rats, Wistar
  • Spinal Cord/cytology

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