Developmental apoptosis in the spinal cord white matter in neonatal rats

A J A De Louw; W D J Van De Berg; J De Vente; C E Blanco; A W D Gavilanes; H P J Steinbusch; H W M Steinbusch; J Troost; J S H Vles

Developmental apoptosis in the spinal cord white matter in neonatal rats

A J A De Louw, W D J Van De Berg, J De Vente, C E Blanco, A W D Gavilanes, H P J Steinbusch, H W M Steinbusch, J Troost, J S H Vles

Research output: Contribution to journal › Article › Academic › peer-review

12 Citations (Scopus)

Abstract

We investigated developmental apoptosis in the white matter of the cervical spinal cord at postnatal days 2, 5, and 8. Apoptotic cells were labeled using TUNEL and caspase-3 immunostaining. Apoptotic cells were diffusely distributed throughout the white matter of the spinal cord. The total amount of apoptotic cells in the cervical spinal cord white matter was related to postnatal age, with the lowest at P2 (mean 7.9, SD 5.6) and the highest at P8 (mean 109, SD 21.4). Using double immunostaining for ED-1 and O4, apoptotic cells were identified as microglia and oligodendrocytes.

Original language	English
Pages (from-to)	89-91
Number of pages	3
Journal	GLIA
Volume	37
Issue number	1
Publication status	Published - Jan 2002

Keywords

Aging/physiology
Animals
Apoptosis/physiology
Caspase 3
Caspases/metabolism
Ectodysplasins
In Situ Nick-End Labeling
Membrane Proteins/metabolism
Microglia/cytology
Nerve Fibers, Myelinated/metabolism
Oligodendroglia/cytology
Rats
Rats, Wistar
Spinal Cord/cytology

Cite this

@article{e11443e6b1b144e789f3e32b213d1ba6,

title = "Developmental apoptosis in the spinal cord white matter in neonatal rats",

abstract = "We investigated developmental apoptosis in the white matter of the cervical spinal cord at postnatal days 2, 5, and 8. Apoptotic cells were labeled using TUNEL and caspase-3 immunostaining. Apoptotic cells were diffusely distributed throughout the white matter of the spinal cord. The total amount of apoptotic cells in the cervical spinal cord white matter was related to postnatal age, with the lowest at P2 (mean 7.9, SD 5.6) and the highest at P8 (mean 109, SD 21.4). Using double immunostaining for ED-1 and O4, apoptotic cells were identified as microglia and oligodendrocytes.",

keywords = "Aging/physiology, Animals, Apoptosis/physiology, Caspase 3, Caspases/metabolism, Ectodysplasins, In Situ Nick-End Labeling, Membrane Proteins/metabolism, Microglia/cytology, Nerve Fibers, Myelinated/metabolism, Oligodendroglia/cytology, Rats, Rats, Wistar, Spinal Cord/cytology",

author = "{De Louw}, {A J A} and {Van De Berg}, {W D J} and {De Vente}, J and Blanco, {C E} and Gavilanes, {A W D} and Steinbusch, {H P J} and Steinbusch, {H W M} and J Troost and Vles, {J S H}",

year = "2002",

month = jan,

language = "English",

volume = "37",

pages = "89--91",

journal = "GLIA",

issn = "0894-1491",

publisher = "John Wiley and Sons Inc.",

number = "1",

}

TY - JOUR

T1 - Developmental apoptosis in the spinal cord white matter in neonatal rats

AU - De Louw, A J A

AU - Van De Berg, W D J

AU - De Vente, J

AU - Blanco, C E

AU - Gavilanes, A W D

AU - Steinbusch, H P J

AU - Steinbusch, H W M

AU - Troost, J

AU - Vles, J S H

PY - 2002/1

Y1 - 2002/1

N2 - We investigated developmental apoptosis in the white matter of the cervical spinal cord at postnatal days 2, 5, and 8. Apoptotic cells were labeled using TUNEL and caspase-3 immunostaining. Apoptotic cells were diffusely distributed throughout the white matter of the spinal cord. The total amount of apoptotic cells in the cervical spinal cord white matter was related to postnatal age, with the lowest at P2 (mean 7.9, SD 5.6) and the highest at P8 (mean 109, SD 21.4). Using double immunostaining for ED-1 and O4, apoptotic cells were identified as microglia and oligodendrocytes.

AB - We investigated developmental apoptosis in the white matter of the cervical spinal cord at postnatal days 2, 5, and 8. Apoptotic cells were labeled using TUNEL and caspase-3 immunostaining. Apoptotic cells were diffusely distributed throughout the white matter of the spinal cord. The total amount of apoptotic cells in the cervical spinal cord white matter was related to postnatal age, with the lowest at P2 (mean 7.9, SD 5.6) and the highest at P8 (mean 109, SD 21.4). Using double immunostaining for ED-1 and O4, apoptotic cells were identified as microglia and oligodendrocytes.

KW - Aging/physiology

KW - Animals

KW - Apoptosis/physiology

KW - Caspase 3

KW - Caspases/metabolism

KW - Ectodysplasins

KW - In Situ Nick-End Labeling

KW - Membrane Proteins/metabolism

KW - Microglia/cytology

KW - Nerve Fibers, Myelinated/metabolism

KW - Oligodendroglia/cytology

KW - Rats

KW - Rats, Wistar

KW - Spinal Cord/cytology

M3 - Article

C2 - 11746787

SN - 0894-1491

VL - 37

SP - 89

EP - 91

JO - GLIA

JF - GLIA

IS - 1

ER -