TY - JOUR
T1 - Dicer-independent processing of small RNA duplexes: mechanistic insights and applications
AU - Herrera-Carrillo, Elena
AU - Berkhout, Ben
PY - 2017
Y1 - 2017
N2 - MicroRNAs (miRNAs) play a pivotal role in the regulation of cellular gene expression via the conserved RNA interference (RNAi) mechanism. Biogenesis of the unusual miR-451 does not require Dicer. This molecule is instead processed by the Argonaute 2 (Ago2) enzyme. Similarly, unconventional short hairpin RNA (shRNA) molecules have been designed as miR-451 mimics that rely exclusively on Ago2 for maturation. We will review recent progress made in the understanding of this alternative processing route. Next, we describe different Dicer-independent shRNA designs that have been developed and discuss their therapeutic advantages and disadvantages. As an example, we will present the route towards development of a durable gene therapy against HIV-1
AB - MicroRNAs (miRNAs) play a pivotal role in the regulation of cellular gene expression via the conserved RNA interference (RNAi) mechanism. Biogenesis of the unusual miR-451 does not require Dicer. This molecule is instead processed by the Argonaute 2 (Ago2) enzyme. Similarly, unconventional short hairpin RNA (shRNA) molecules have been designed as miR-451 mimics that rely exclusively on Ago2 for maturation. We will review recent progress made in the understanding of this alternative processing route. Next, we describe different Dicer-independent shRNA designs that have been developed and discuss their therapeutic advantages and disadvantages. As an example, we will present the route towards development of a durable gene therapy against HIV-1
U2 - https://doi.org/10.1093/nar/gkx779
DO - https://doi.org/10.1093/nar/gkx779
M3 - Article
C2 - 28977573
SN - 0305-1048
VL - 45
SP - 10369
EP - 10379
JO - Nucleic Acids Research
JF - Nucleic Acids Research
IS - 18
ER -