Differentiation and proliferation of respiration-deficient human myoblasts

N. H. Herzberg, R. Zwart, R. A. Wolterman, J. P. Ruiter, R. J. Wanders, P. A. Bolhuis, C. van den Bogert

Research output: Contribution to JournalArticleAcademicpeer-review

60 Citations (Scopus)

Abstract

Replication and transcription of mitochondrial DNA were impaired in dividing human myoblasts exposed to ethidium bromide. MtDNA content decreased linearly per cell division and mitochondrial transcript levels declined rapidly, resulting in respiration-deficiency of the myoblasts. Despite the absence of functional mitochondria the cells remained able to proliferate when grown under specific culture conditions. However, the formation of myotubes was severely impaired in respiration-deficient myoblasts. We conclude that differentiation of myoblasts into myotubes is more dependent on mitochondrial function than proliferation of myoblasts
Original languageEnglish
Pages (from-to)63-67
JournalBIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
Volume1181
Issue number1
DOIs
Publication statusPublished - 1993

Cite this