TY - JOUR
T1 - Early inflammatory response during the development of right ventricular heart failure in a rat model
AU - Campian, Maria E.
AU - Hardziyenka, Maxim
AU - de Bruin, Kora
AU - van Eck-Smit, Berthe L. F.
AU - de Bakker, Jacques M. T.
AU - Verberne, Hein J.
AU - Tan, Hanno L.
PY - 2010
Y1 - 2010
N2 - Inflammatory activation plays an important role in the pathogenesis and progression of left ventricular (LV) heart failure. In right ventricular (RV) heart failure, little is known about the role of inflammatory activation. We aimed to study the role of inflammatory activation in RV heart failure by serial monitoring during disease progression. Right ventricular heart failure was induced in male Wistar rats by intraperitoneal injection of monocrotaline (MCT). Two groups were studied: MCT-treated rats (MCT-rats), and age-matched controls (CON-rats). Serial echocardiography and in vivo 67-Gallium (Ga-67) scintigraphy were performed. Local inflammation in the RV was assessed by (i) ex vivo semi-quantitative Ga-67 autoradiography, (ii) immunohistochemistry of myeloperoxidase (MPO), a marker of neutrophil activity, and (iii) mRNA assays of tumour necrosis factor-alpha (TNF-alpha). In MCT-rats, Ga-67 scintigraphy showed increased myocardial uptake which started during the early stages of RV disease. Ga-67 autoradiography revealed that this increased Ga-67 uptake occurred in the RV and inter-ventricular septum, but not in the LV. The stage-dependent increases of in vivo Ga-67 RV myocardial uptake were paralleled by increases in mRNA gene expression for TNF-alpha in RV, and increased MPO staining in RV. Development and progression of RV heart failure is associated with an early increase in RV inflammation. Ga-67 scintigraphy may be used for the serial assessment of inflammation and monitoring of disease progression in RV heart failure
AB - Inflammatory activation plays an important role in the pathogenesis and progression of left ventricular (LV) heart failure. In right ventricular (RV) heart failure, little is known about the role of inflammatory activation. We aimed to study the role of inflammatory activation in RV heart failure by serial monitoring during disease progression. Right ventricular heart failure was induced in male Wistar rats by intraperitoneal injection of monocrotaline (MCT). Two groups were studied: MCT-treated rats (MCT-rats), and age-matched controls (CON-rats). Serial echocardiography and in vivo 67-Gallium (Ga-67) scintigraphy were performed. Local inflammation in the RV was assessed by (i) ex vivo semi-quantitative Ga-67 autoradiography, (ii) immunohistochemistry of myeloperoxidase (MPO), a marker of neutrophil activity, and (iii) mRNA assays of tumour necrosis factor-alpha (TNF-alpha). In MCT-rats, Ga-67 scintigraphy showed increased myocardial uptake which started during the early stages of RV disease. Ga-67 autoradiography revealed that this increased Ga-67 uptake occurred in the RV and inter-ventricular septum, but not in the LV. The stage-dependent increases of in vivo Ga-67 RV myocardial uptake were paralleled by increases in mRNA gene expression for TNF-alpha in RV, and increased MPO staining in RV. Development and progression of RV heart failure is associated with an early increase in RV inflammation. Ga-67 scintigraphy may be used for the serial assessment of inflammation and monitoring of disease progression in RV heart failure
U2 - https://doi.org/10.1093/eurjhf/hfq066
DO - https://doi.org/10.1093/eurjhf/hfq066
M3 - Article
C2 - 20495202
SN - 1388-9842
VL - 12
SP - 653
EP - 658
JO - European journal of heart failure
JF - European journal of heart failure
IS - 7
ER -