TY - JOUR
T1 - Effect of endotoxin on permeability of bovine cerebral endothelial cell layers in vitro
AU - De Vries, Helga E.
AU - Blom-Roosemalen, Margret C.M.
AU - De Boer, Albertus G.
AU - Van Berkel, Theo J.C.
AU - Breimer, Douwe D.
AU - Kuiper, Johan
PY - 1996/6
Y1 - 1996/6
N2 - The effect of lipopolysaccharide (LPS) on cultured cerebral endothelial cells was investigated to assess the changes in the trans endothelial electrical resistance (TEER) across the blood-brain barrier that may occur during inflammatory diseases of the central nervous system. Primary cultures of bovine cerebral endothelial cells were cultured to tight monolayers with a TEER of 250 to 300 Ω · cm2 on polycarbonate Transwell filters. LPS induced a time- and dose-dependent decline in TEER. Transport of the hydrophilic model compounds sodium fluorescein and fluorescein dextran (M(R), 4 kDa) across monolayers of bovine cerebral endothelial cells increased more than 3- fold after treatment of the cells with LPS (50 ng/ml). Treatment of the monolayers with various concentrations of LPS caused a 3-to 4-fold increase in the permeability of bovine cerebral endothelial cells for [125I]bovine serum albumin, which was also preceded by a decrease in TEER. The reduction of TEER by LPS could be inhibited completely by indomethacin (10-6 M for 30 min), a cyclooxygenase inhibitor, but not by dexamethasone, a glucocorticoid (10-7 M for 16 hr). In conclusion, LPS administration to blood-brain barrier endothelial cells causes a decrease in TEER which leads to enhanced transport of low and high molecular weight molecules. During this process the production of eicosanoids by the endothelial cells seem to play a key role.
AB - The effect of lipopolysaccharide (LPS) on cultured cerebral endothelial cells was investigated to assess the changes in the trans endothelial electrical resistance (TEER) across the blood-brain barrier that may occur during inflammatory diseases of the central nervous system. Primary cultures of bovine cerebral endothelial cells were cultured to tight monolayers with a TEER of 250 to 300 Ω · cm2 on polycarbonate Transwell filters. LPS induced a time- and dose-dependent decline in TEER. Transport of the hydrophilic model compounds sodium fluorescein and fluorescein dextran (M(R), 4 kDa) across monolayers of bovine cerebral endothelial cells increased more than 3- fold after treatment of the cells with LPS (50 ng/ml). Treatment of the monolayers with various concentrations of LPS caused a 3-to 4-fold increase in the permeability of bovine cerebral endothelial cells for [125I]bovine serum albumin, which was also preceded by a decrease in TEER. The reduction of TEER by LPS could be inhibited completely by indomethacin (10-6 M for 30 min), a cyclooxygenase inhibitor, but not by dexamethasone, a glucocorticoid (10-7 M for 16 hr). In conclusion, LPS administration to blood-brain barrier endothelial cells causes a decrease in TEER which leads to enhanced transport of low and high molecular weight molecules. During this process the production of eicosanoids by the endothelial cells seem to play a key role.
UR - http://www.scopus.com/inward/record.url?scp=0030433677&partnerID=8YFLogxK
M3 - Article
C2 - 8667205
SN - 0022-3565
VL - 277
SP - 1418
EP - 1423
JO - Journal of Pharmacology and Experimental Therapeutics
JF - Journal of Pharmacology and Experimental Therapeutics
IS - 3
ER -